Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins

doi:10.1016/j.jprot.2014.06.022

	Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins
	An, Bang 1; Chen, Yong 1; Li, Boqiang ; Qin, Guozheng; Tian, Shiping
	2014
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	109 页码:38-49
摘要	Reactive oxygen species (ROS) play a vital role in reducing viability of yeast cells. The Ca2+-CaM signaling pathways are involved in regulating the intracellular ROS level in yeast cells under stress. Detergent resistant membranes (DRMs), the sterol-rich microdomains, participate in a wide range of cellular processes including growth, trafficking and death in yeast cells. In the present study, we found that Trifluoperazine (TFP), an antagonist of CaM, could increase the viability of Candida guilliermondii cells under H2O2 stress. Based on comparative analysis of DRM sub proteomics, a total number of 29 differentially expressed protein spots were identified, among which 8 protein spots belong to the electron transport chain and 7 protein spots belong to transporters. It is suggested that TFP treatment could modulate the intracellular ROS generation in yeast cells. We additionally ascertained that TFP treatment could effectively alleviate the ROS accumulation and protein damage in C. guilliermondii cells under H2O2 stress, via investigating the intracellular ROS levels and protein oxidative damage in yeast cells. These findings firstly revealed that the Ca2+-CaM signaling pathway is related to the viability of yeast cells under H2O2 stress, and provide novel evidences for exploring Ca2+-CaM's role in regulating this viability via acting on DRM proteins. Biological significance Detergent-resistant membranes (DRMs), which are more resistant to extraction with cold non-ionic detergents, have been considered the functional microdomains in the plasma membrane. In yeast, DRMs are involved in a wide range of additional cellular processes including cell growth and death. The Ca2+-CaM signaling pathways could regulate the stress tolerance of yeast cells by modulating the intracellular ROS generation. In this study, we found that trifluoperazine (TFP), a calmodulin antagonist, could increase the viability of C. guilliermondii under H2O2 stress. Based on comparative analysis of DRM sub proteomics, electron transport chain proteins and transporters were identified to be associated with the Ca2+-CaM transduction. We proved that TFP treatment decreases the intracellular ROS accumulation and alleviates oxidative damage to cellular proteins. These results ascertain that Ca2+-CaM is involved in regulating the viability of C. guilliermondii under oxidative stress via acting on the DRM proteins. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Ca2+-CaM Detergent resistant membrane Subproteomics Oxidative stress Biocontrol microbial agent
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2014.06.022
收录类别	SCI
语种	英语
WOS关键词	DEPENDENT ANION CHANNEL ; LIPID RAFTS ; SACCHAROMYCES-CEREVISIAE ; CYTOCHROME-C ; YEAST ; APOPTOSIS ; COMPLEX ; CALCIUM ; DOMAINS ; TRIFLUOPERAZINE
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000345542600004
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National High Technology Research (863) Program of China [2012AA101607] ; National Natural Science Foundation of China [31030051, 31172005]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27193
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	An, Bang,Chen, Yong,Li, Boqiang,et al. Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins[J]. JOURNAL OF PROTEOMICS,2014,109:38-49.
APA	An, Bang,Chen, Yong,Li, Boqiang,Qin, Guozheng,&Tian, Shiping.(2014).Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins.JOURNAL OF PROTEOMICS,109,38-49.
MLA	An, Bang,et al."Ca2+-CaM regulating viability of Candida guilliermondii under oxidative stress by acting on detergent resistant membrane proteins".JOURNAL OF PROTEOMICS 109(2014):38-49.

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