Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis

doi:10.1021/pr500337j

	Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis
	Wang, Zizhang; Xue, Zhen; Wang, Tai
	2014
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	13 期号:9 页码:3987-4001
摘要	A century ago, dominance and overdominance hypotheses were developed to explain the phenomenon of heterosis, both hypotheses were in a nonadditive pattern. Here, a principal component analysis (PCA) of maize seed proteomes was used for representative inbreds of five heterotic germplasms and three classes of hybrid. Hybrids congregated in the center region of inbreds, forming an additive distribution with hybrids in the middle of their parents. Principal components 1 and 2 indicated biased distributions of proteins with functions of amino acid protein or carbohydrate energy metabolisms, respectively, after loading analysis and MS identification of proteins. Then, GC MS was used to examine free amino acids, carbohydrates, and organic acids. A lower level of these metabolites were found in hybrids than inbreds. Further, we performed similar analyses of germinating seeds of a parent-F1 triad and three F2 segregants and confirmed these results. Therefore, an additive pattern of protein abundances for an unimpeded flow of metabolites was established in heterotic hybrids. That is, an additively balanced networking but not the nonadditive dominance or overdominance regulates heterosis. The less expensive metabolism in hybrids suggested the evolution of sexual reproduction. The Mendelian phenotypic ratio can be better explained based on this additive pattern than dominance.
关键词	Proteomics metabolomics principal component analysis additively balanced networking heterosis maize
学科领域	Biochemical Research Methods
DOI	10.1021/pr500337j
收录类别	SCI
语种	英语
WOS关键词	NONADDITIVE PROTEIN ACCUMULATION ; MAYS L. F-1-HYBRID ; GENE-EXPRESSION ; INBRED LINES ; GROWTH ; PATTERNS ; RICE ; HETEROZYGOSITY ; DIVERSITY ; HYBRIDS
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000341345000012
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
EISSN	1535-3907
资助机构	China Ministry of Agriculture [2008-2013ZX08012-002]
作者邮箱	twang@ibcas.ac.cn
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27198
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zizhang,Xue, Zhen,Wang, Tai. Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis[J]. JOURNAL OF PROTEOME RESEARCH,2014,13(9):3987-4001.
APA	Wang, Zizhang,Xue, Zhen,&Wang, Tai.(2014).Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis.JOURNAL OF PROTEOME RESEARCH,13(9),3987-4001.
MLA	Wang, Zizhang,et al."Differential Analysis of Proteomes and Metabolomes Reveals Additively Balanced Networking for Metabolism in Maize Heterosis".JOURNAL OF PROTEOME RESEARCH 13.9(2014):3987-4001.

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