A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo

doi:10.1111/ppl.12235

	A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo
	Jia, Husen ; Dwyer, Simon A.; Fan, Da-Yong ; Han, Yaqin ; Badger, Murray R.; von Caemmerer, Susanne ; Chow, Wah Soon 6
	2014
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	152 期号:3 页码:403-413
摘要	We sought a rapid, non-intrusive, whole-tissue measure of the functional photosystem II (PS II) content in leaves. Summation of electrons, delivered by a single-turnover flash to P700(+) (oxidized PS I primary donor) in continuous background far-red light, gave a parameter S in absorbance units after taking into account an experimentally determined basal electron flux that affects P700 redox kinetics. S was linearly correlated with the functional PS II content measured by the O-2 yield per single-turnover repetitive flash in Arabidopsis thaliana expressing an antisense construct to the PsbO (manganese-stabilizing protein in PS II) proteins of PS II (PsbO mutants). The ratio of S to z(max) (total PS I content in absorbance units) was comparable to the PS II/PS I reaction-center ratio in wild-type A. thaliana and in control Spinacea oleracea. Both S and S/z(max) decreased in photoinhibited spinach leaf discs. The whole-tissue functional PS II content and the PS II/photosystem I (PS I) ratio can be non-intrusively monitored by S and S/z(max), respectively, using a quick P700 absorbance protocol compatible with modern P700 instruments.
学科领域	Plant Sciences
DOI	10.1111/ppl.12235
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYNTHETIC ELECTRON-TRANSPORT ; ABSORBENCY CHANGES ; CHLOROPHYLL FLUORESCENCE ; STROMAL COMPONENTS ; INTACT LEAVES ; QUANTUM YIELD ; CHLOROPLASTS ; PHOTOINHIBITION ; FLASH ; STATE
WOS研究方向	Plant Sciences
WOS记录号	WOS:000343972500001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1399-3054
资助机构	Australian Postgraduate Award ; Chinese Academy of Sciences [KZCX2-XB3-09-02] ; Chinese Academy of Sciences (NNSF of China) [31370424] ; Australian Research Council [DP1093827, CE056159]
作者邮箱	Fred.Chow@anu.edu.au
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27201
专题	植被与环境变化国家重点实验室
作者单位	1.[Jia, Husen 2.Dwyer, Simon A. 3.Fan, Da-Yong 4.Han, Yaqin 5.Badger, Murray R. 6.von Caemmerer, Susanne 7.Australian Natl Univ, Coll Med Biol & Environm, Res Sch Biol, Div Plant Sci, Canberra, ACT, Australia 8.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Jia, Husen,Dwyer, Simon A.,Fan, Da-Yong,et al. A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo[J]. PHYSIOLOGIA PLANTARUM,2014,152(3):403-413.
APA	Jia, Husen.,Dwyer, Simon A..,Fan, Da-Yong.,Han, Yaqin.,Badger, Murray R..,...&Chow, Wah Soon.(2014).A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo.PHYSIOLOGIA PLANTARUM,152(3),403-413.
MLA	Jia, Husen,et al."A novel P700 redox kinetics probe for rapid, non-intrusive and whole-tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo".PHYSIOLOGIA PLANTARUM 152.3(2014):403-413.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Physiologia Plantaru（860KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文