Knowledge Management System Of Institute Of Botany,CAS
| Large-scale analysis of protein phosphorylation in Populus leaves | |
| Liu, Jinwen1; Ning, Deli; Zhao, Guiling2; Cheng, Yuxiang; Wang, Baichen3 | |
| 2014 | |
| 发表期刊 | JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| ISSN | 0971-7811 |
| 卷号 | 23期号:4页码:410-420 |
| 摘要 | Protein phosphorylation is a key regulatory factor in all aspects of plant biology; most regulatory pathways are governed by the reversible phosphorylation of proteins. To better understand the role that phosphorylated proteins play in a woody model plant, we performed a systemic analysis of the phosphoproteome from Populus leaves using high accuracy NanoLC-MS/MS in combination with biochemical enrichments using strong cation exchange chromatography and titanium dioxide chromatography. We identified 104 phosphopeptides from 94 phosphoproteins and determined 111 phosphorylation sites including 93 occurring on serine residues and 18 on threonine residues. The identified phosphoproteins are involved in a wide variety of metabolic processes. Among these identified phosphoproteins, 68 phosphorylation sites (72 %) were located outside of conserved domains. The identified phosphopeptides share a common phosphorylation motif of pS/pT-P/D-S/A. These data suggest that the Populus metabolism and gene regulation machinery are major targets of phosphorylation. To our knowledge, this is the first gel-free, large-scale phosphoproteomics analysis in woody plants. The identified phosphorylation sites will be a valuable resource for many fields of plant biology, and information gained from the study will provide a better understanding of protein phosphorylation. |
| 关键词 | Phosphoproteomics Populus simonii x Populus nigra Posttraslational modification |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences |
| DOI | 10.1007/s13562-013-0225-7 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | TYROSINE PHOSPHORYLATION ; PHOSPHOPROTEOME ANALYSIS ; MASS-SPECTROMETRY ; IN-VIVO ; QUANTITATIVE PHOSPHOPROTEOMICS ; THYLAKOID MEMBRANES ; INITIATION-FACTORS ; PLASMA-MEMBRANE ; PLANT ; REVEALS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000343163400009 |
| 出版者 | SPRINGER INDIA |
| 文献子类 | Article |
| 出版地 | NEW DELHI |
| EISSN | 0974-1275 |
| 资助机构 | State Key Program of National Natural Science of China [31030017] ; National Basic Research Priorities Programme [2009CB119102] ; Fundamental Research Funds for the Central Universities [DL09EA01] ; Natural Sciences Foundation of Heilongjiang Province [C201010] ; Northeast Forestry University [Grap 09] |
| 作者邮箱 | chengyuxiang@nefu.edu.cn ; wangbc@ibcas.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/27203 |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Northeast Forestry Univ, State Key Lab Forest Genet & Tree Breeding, Harbin 150040, Peoples R China 2.Heilongjiang Bayi Agr Univ, Coll Life Sci & Biotechnol, Daqing 163319, Peoples R China 3.Shenyang Agr Univ, Coll Forestry, Shenyang 110866, Liaoning, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jinwen,Ning, Deli,Zhao, Guiling,et al. Large-scale analysis of protein phosphorylation in Populus leaves[J]. JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY,2014,23(4):410-420. |
| APA | Liu, Jinwen,Ning, Deli,Zhao, Guiling,Cheng, Yuxiang,&Wang, Baichen.(2014).Large-scale analysis of protein phosphorylation in Populus leaves.JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY,23(4),410-420. |
| MLA | Liu, Jinwen,et al."Large-scale analysis of protein phosphorylation in Populus leaves".JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 23.4(2014):410-420. |
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