The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution

doi:10.1016/j.jplph.2013.11.008

IB-CAS > 中科院光生物学重点实验室

	The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution
	Zhang, Lei 1; Melo, Thor Bernt ; Li, Heng ; Naqvi, K. Razi ; Yang, Chunhong
	2014
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	171 期号:5 页码:42-48
摘要	Under strong light conditions, long-lived chlorophyll triplets ((3)Chls) are formed, which can sensitize singlet oxygen, a species harmful to the photosynthetic apparatus of plants. Plants have developed multiple photoprotective mechanisms to quench (3)Chl and scavenge singlet oxygen in order to sustain the photosynthetic activities. The lumenal loop of light-harvesting chlorophyll a/b complex of photosystem II (LHCII) plays important roles in regulating the pigment conformation and energy dissipation. In this study, site-directed mutagenesis analysis was applied to investigate triplet-triplet energy transfer and quenching of (3)Chl in LHCII. We mutated the amino acid at site 123 located in this region to Gly, Pro, Gln, Thr and Tyr, respectively, and recorded fluorescence excitation spectra, triplet-minus-singlet (TmS) spectra and kinetics of carotenoid triplet decay for wild type and all the mutants. A red-shift was evident in the TmS spectra of the mutants S123T and S123P, and all of the mutants except S123Y showed a decrease in the triplet energy transfer efficiency. We propose, on the basis of the available structural information, that these phenomena are related to the involvement, due to conformational changes in the lumenal region, of a long-wavelength lutein (Lut2) involved in quenching (3)Chl. (C) 2013 Elsevier GmbH. All rights reserved.
关键词	Light-harvesting chlorophyll a/b complexes Conformation Chlorophyll triplet Lutein Mutagenesis
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2013.11.008
收录类别	SCI
语种	英语
WOS关键词	HIGHER-PLANTS ; BINDING ; XANTHOPHYLLS ; MEMBRANES ; DYNAMICS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000331925900007
出版者	ELSEVIER GMBH, URBAN & FISCHER VERLAG
文献子类	Article
出版地	JENA
EISSN	1618-1328
资助机构	State Key Basic Research and Development Plan of China [2011CBA00904] ; National Natural Science Foundation of China [31070212, 31370275] ; Research Council of Norway [191102]
作者邮箱	razi.naqvi@ntnu.no ; yangch@ibcas.ac.cn
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27212
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Norwegian Univ Sci & Technol, Dept Phys, N-7491 Trondheim, Norway
推荐引用方式 GB/T 7714	Zhang, Lei,Melo, Thor Bernt,Li, Heng,et al. The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution[J]. JOURNAL OF PLANT PHYSIOLOGY,2014,171(5):42-48.
APA	Zhang, Lei,Melo, Thor Bernt,Li, Heng,Naqvi, K. Razi,&Yang, Chunhong.(2014).The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution.JOURNAL OF PLANT PHYSIOLOGY,171(5),42-48.
MLA	Zhang, Lei,et al."The inter-monomer interface of the major light-harvesting chlorophyll a/b complexes of photosystem II (LHCII) influences the chlorophyll triplet distribution".JOURNAL OF PLANT PHYSIOLOGY 171.5(2014):42-48.

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