Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony

doi:10.1093/aob/mct273

	Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony
	Hao, Hai-ping ; He, Zhi ; Li, Hui ; Shi, Lei; Tang, Yu-Dan
	2014
发表期刊	ANNALS OF BOTANY
ISSN	0305-7364
卷号	113 期号:3 页码:443-452
摘要	Epicotyl dormancy break in seeds that have deep simple epicotyl morphophysiological dormancy (MPD) requires radicle emergence and even a certain root length in some species. However, the mechanisms by which root length affects epicotyl dormancy break are not clear at present. This study aims to explore the relationship between root length and epicotyl dormancy release in radicle-emerged seeds of Tibetan peony, Paeonia ludlowii, with discussion of the possible mechanisms. Radicle-emerged seeds (radicle length 15, 30, 45 and 60 cm) were incubated at 5, 10 and 15 C. During the stratification, some seeds were transferred to 15 C and monitored for epicotylplumule growth. Hormone content was determined by ELISA, and the role of hormones in epicotyl dormancy release was tested by exogenous hormone and embryo culture. Cold stratification did not break the epicotyl dormancy until the root length was 6 cm. The indole-3-actic acid (IAA) and GA(3) contents of seeds having 6 cm roots were significantly higher than those of seeds with other root lengths, but the abscisic acid (ABA) content was lowest among radicle-emerged seeds. GA(3) (400 mg L-1) could break epicotyl dormancy of all radicle-emerged seeds, while IAA (200 mg L-1) had little or no effect. When grown on MS medium, radicles of naked embryos grew and cotyledons turned green, but epicotyls did not elongate. Naked embryos developed into seedlings on a mixed medium of MS 100 mg L-1 GA(3). A root length of 60 cm is necessary for epicotyl dormancy release by cold stratification. The underlying reason for root length affecting epicotyl dormancy release is a difference in the GA(3)/ABA ratio in the epicotyl within radicle-emerged seeds, which is mainly as a result of a difference in ABA accumulation before cold stratification.
关键词	Seed epicotyl dormancy release hormone root length Paeonia ludlowii Tibetan peony
学科领域	Plant Sciences
DOI	10.1093/aob/mct273
收录类别	SCI
语种	英语
WOS关键词	ENDOGENOUS ABSCISIC-ACID ; ARABIDOPSIS-THALIANA ; GERMINATION ; MAINTENANCE ; DEFICIENT
WOS研究方向	Plant Sciences
WOS记录号	WOS:000331265200005
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1095-8290
资助机构	National Key Technology RD Program [2012BAC01B05] ; Knowledge Innovation Engineering of the Chinese Academy of Sciences [KSCX2-EW-B-5] ; Strategic Technical Support System of Special Biological Resources Project [CZBZX-1]
作者邮箱	shilei67@263.net ; tangyudan@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27215
专题	中科院北方资源植物重点实验室
作者单位	1.[Hao, Hai-ping 2.He, Zhi 3.Li, Hui 4.Shi, Lei 5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Hao, Hai-ping,He, Zhi,Li, Hui,et al. Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony[J]. ANNALS OF BOTANY,2014,113(3):443-452.
APA	Hao, Hai-ping,He, Zhi,Li, Hui,Shi, Lei,&Tang, Yu-Dan.(2014).Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony.ANNALS OF BOTANY,113(3),443-452.
MLA	Hao, Hai-ping,et al."Effect of root length on epicotyl dormancy release in seeds of Paeonia ludlowii, Tibetan peony".ANNALS OF BOTANY 113.3(2014):443-452.

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