Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica

doi:10.1016/j.cryobiol.2014.03.002

	Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica
	Li, Yang; Shi, Lei
	2014
发表期刊	CRYOBIOLOGY
ISSN	0011-2240
卷号	68 期号:3 页码:446-450
摘要	In order to effectively preserve green spores, which have relatively higher water content and lose viability more quickly than non-green spores, we studied the effect of desiccation level and storage temperature on Osmunda japonica spores. The water content of fresh spores was 11.20%. After 12 h desiccation by silica gel, the water content decreased to 6% but spore viability did not change significantly. As the desiccation continued, the decrease in water content slowed, but spore viability dropped. For almost all storage periods, the effects of storage temperature, desiccation level, and temperature x desiccation level were significantly different. After seven days of storage, spores at any desiccation level stored at 4 degrees C obtained high germination rates. After more than seven days storage, liquid nitrogen (LN) storage obtained the best results. Storage at 18 degrees C led to the lowest germination rates. Spores stored at room temperature and 18 degrees C all died within three months. For storage at 4 degrees C and in LN, spores desiccated 12 and 36 h obtained better results. Spores without desiccation had the highest germination rates after being stored at room temperature, but suffered the greatest loss after storage at 18 degrees C. These results suggest that LN storage is the best method of long-term storage of O. japonica spores. The critical water content of O. japonica spores is about 6% and reduction of the water content to this level improves outcome after LN storage greatly. The reason for various responses of O. japonica spores to desiccation and storage temperatures are discussed. (C) 2014 Elsevier Inc. All rights reserved.
关键词	Osmunda japonica Green spore Liquid nitrogen (LN) storage Desiccation Spore viability
学科领域	Biology ; Physiology
DOI	10.1016/j.cryobiol.2014.03.002
收录类别	SCI
语种	英语
WOS关键词	FERN SPORES ; EMBRYONIC AXES ; CRYOPRESERVATION ; SEEDS ; SURVIVAL ; WATER ; CONSERVATION ; GERMINATION ; HYPOTHESIS ; MOISTURE
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Physiology
WOS记录号	WOS:000336884100019
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
文献子类	Article
出版地	SAN DIEGO
EISSN	1090-2392
资助机构	National Key Technology Support Program of the Ministry of Science and Technology of China [2012BAC01B05] ; Knowledge Innovative Program of The Chinese Academy of Sciences [KSCX2-EW-B-5] ; Special Program for Technology Supporting System of Strategic Biological Resources In Vitro Plant Germplasm Collection, BRC-CAS [CZBZX-1]
作者邮箱	liyang1@ibcas.ac.cn ; chuanxia97420@163.com
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27217
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Li, Yang,Shi, Lei. Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica[J]. CRYOBIOLOGY,2014,68(3):446-450.
APA	Li, Yang,&Shi, Lei.(2014).Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica.CRYOBIOLOGY,68(3),446-450.
MLA	Li, Yang,et al."Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica".CRYOBIOLOGY 68.3(2014):446-450.

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