Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea

doi:10.1016/j.plantsci.2014.06.018

	Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea
	Cao, Di ; Stewart, C. Neal, Jr.; Zheng, Min ; Guan, Zhengjun ; Tang, Zhi-xi ; Wei, Wei ; Ma, Ke-ping
	2014
发表期刊	PLANT SCIENCE
ISSN	0168-9452
卷号	227 页码:45-50
摘要	Transgenic canola (Brassica napus) with a Bacillus thuringiensis cry1Ac gene and a green fluorescent protein (GFP) marker gene was used in hybridization experiments with wild Brassica juncea. Hybrid F-1 and successive five backcross generations were obtained. The pod-set frequency on backcrossed B. juncea plants was over 66%, which suggested relatively high crossing compatibility between the hybrids and wild species. The seed setting in BC1 was the least of all generations tested, and then increased at the BC2 generation for which the thousand-seed weight was the highest of all generations. Seed size in backcrossed generations eventually approached that of the wild parent. The plants in all backcrossed generations were consistent with the expected 1:1 segregation ratio of the transgenes. The Bt Cry1Ac protein concentrations at bolting and flowering stages was higher compared to the 4-5-leaf and pod-formation stages. Nonetheless, the Bt toxin in the fifth backcrossing generation (BC5) was sufficient to kill both polyphagous (Helicoverpa armigera) and oligophagous (Plutella xylostella) Lepidoptera. As a consequence, the subsequent generations harboring the transgene from F-1 to BC5 could have selection advantage against insect pests. The result is useful in understanding gene flow from transgenic crops and the followed transgene introgression into wild. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
关键词	Brassica napus Bt Cry1Ac protein Gene flow Insect bioassay Introgression Segregation ratio
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1016/j.plantsci.2014.06.018
收录类别	SCI
语种	英语
WOS关键词	GREEN-FLUORESCENT PROTEIN ; OILSEED RAPE ; HERBICIDE RESISTANCE ; BT TRANSGENES ; HYBRIDS ; EXPRESSION ; HYBRIDIZATION ; FITNESS ; POPULATIONS ; BACKCROSS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000342865000006
出版者	ELSEVIER IRELAND LTD
文献子类	Article
出版地	CLARE
资助机构	National Natural Science Foundation of China [31270578, 31200422, 31370357] ; Commonweal Scientific Program of Environmental Protection [201109028]
作者邮箱	weiwei@ibcas.ac.cn
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27221
专题	植被与环境变化国家重点实验室
作者单位	1.[Cao, Di 2.Zheng, Min 3.Guan, Zhengjun 4.Tang, Zhi-xi 5.Wei, Wei 6.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 7.[Stewart, C. Neal, Jr.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA
推荐引用方式 GB/T 7714	Cao, Di,Stewart, C. Neal, Jr.,Zheng, Min,et al. Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea[J]. PLANT SCIENCE,2014,227:45-50.
APA	Cao, Di.,Stewart, C. Neal, Jr..,Zheng, Min.,Guan, Zhengjun.,Tang, Zhi-xi.,...&Ma, Ke-ping.(2014).Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea.PLANT SCIENCE,227,45-50.
MLA	Cao, Di,et al."Stable Bacillus thuringiensis transgene introgression from Brassica napus to wild mustard B. juncea".PLANT SCIENCE 227(2014):45-50.

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