The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.

doi:10.1016/j.jprot.2014.01.029

	The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.
	Shen, Hongling 1; He, Zhenyan; Yan, Huili 1; Xing, Zenan 1; Chen, Yanshan 1; Xu, Wenxiu ; Xu, Wenzhong ; Ma, Mi
	2014
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	105 页码:46-57
摘要	Pteris vittata, the first known arsenic hyperaccumulating plant, can accumulate very high concentration arsenic in its aboveground tissues, while low in roots. Previous studies have suggested that arsenic vacuole compartmentalization may play an important role in the arsenic-hyperaccumulation in P. vittata, but the mechanism(s) of arsenic transport to vacuole are largely unknown. We obtained tonoplast isolated from fronds of P. vittata sporophyte grown under minus and 1 mM arsenate for 3 weeks by iodixanol step gradient centrifugation method, and then used TMPP protein labeling technology followed by liquid chromatography a linear ion trap-Orbitrap hybrid mass spectrometer analysis for the quantitative detection of proteins. And we designed and used an artificial database for database searching. In total, 56 tonoplast proteins were identified; more than 70% of them were transport proteins. Under arsenate treatment, one TDT transporter protein, a member of the TerC family and a PDR-like protein were upregulated differentially. While V-ATPase subunits c, E, and G, and V-PPase, were downregulated. Additionally, the identified tonoplast proteins in our present study provide an informative basis for arsenic carriers or channels and help to clarify the regulation of tonoplast arsenic transport processes in P. vittata. Biological significance Vacuole compartmentalization is crucial to As hyperaccumulator P. vittata, while there is limited known arsenic transport proteins involved in vacuole compartmentalization. In this paper, we obtained tonoplast of P. vittata fronds by iodixanol step gradient centrifugation method and then used TMPP protein labeling proteome technology for the quantitative detection of fronds tonoplast proteins. Our findings are the first challenge to the tonoplast proteins data mining of P. vittata which provide an informative basis for As carriers or channels. The proteomic approach in our study is suited for detecting alterations tonoplast protein and help to clarify the regulation of tonoplast transport processes. This article is part of a Special Issue entitled: Proteomics of non-model organisms. (C) 2014 Elsevier B.V. All rights reserved.
关键词	Pteris vittata Vacuole transporter Compartmentalization Arsenic detoxification Membrane proteomic
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2014.01.029
收录类别	SCI
语种	英语
WOS关键词	EMBRYONIC STEM-CELLS ; SACCHAROMYCES-CEREVISIAE ; OXIDATIVE STRESS ; CHINESE BRAKE ; ARABIDOPSIS ; TRANSPORTER ; TOLERANCE ; MECHANISMS ; SPECIATION ; ATPASE
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000338600000005
出版者	ELSEVIER
Special Issue	SI
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National Natural Science Foundation of China [31070449, 31370299]
作者邮箱	hezhenyan@ibcas.ac.cn
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27226
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Shen, Hongling,He, Zhenyan,Yan, Huili,et al. The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.[J]. JOURNAL OF PROTEOMICS,2014,105:46-57.
APA	Shen, Hongling.,He, Zhenyan.,Yan, Huili.,Xing, Zenan.,Chen, Yanshan.,...&Ma, Mi.(2014).The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L..JOURNAL OF PROTEOMICS,105,46-57.
MLA	Shen, Hongling,et al."The fronds tonoplast quantitative proteomic analysis in arsenic hyperaccumulator Pteris vittata L.".JOURNAL OF PROTEOMICS 105(2014):46-57.

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