Mechanism of Penicillium expansum in response to exogenous nitric oxide based on proteomics analysis

doi:10.1016/j.jprot.2014.03.012

	Mechanism of Penicillium expansum in response to exogenous nitric oxide based on proteomics analysis
	Lai, Tongfei; Chen, Yong; Li, Boqiang; Qin, Guozheng; Tian, Shiping
	2014
发表期刊	JOURNAL OF PROTEOMICS
ISSN	1874-3919
卷号	103 页码:47-56
摘要	Penicillium expansurn is an important fungal pathogen, which causes blue mold rot in various fruits and produces a mycotoxin (patulin) with potential damage to public health. Here, we found that nitric oxide (NO) donor could significantly inhibit germinability of P. expansum spores, resulting in lower virulence to apple fruit. Based on two dimension electrophoresis (2-DE) and mass spectrometry (MS) analysis, we identified ten differentially expressed proteins in response to exogenous NO in P. expansum. Among of them, five proteins, such as glutamine synthetase (GS), amidohydrolase, nitrilases, nitric oxide dioxygenase (NOD) and heat shock protein 70, were up-regulated. Others including tetratricopeptide repeat domain, UDP-N-acetylglucosamine pyrophosphorylase, enolase (Eno), heat shock protein 60 and K homology RNA-binding domain were down-regulated. The expression of three genes associated with the identified proteins (GS, NOD, and Eno) was evaluated at the mRNA level by RT-PCR. Our results provide the novel evidence for understanding the mechanism, by which NO regulates growth of P. expansum and its virulence. Biological significance Crop diseases caused by fungal pathogens lead to huge economic losses every year in the world. Application of chemical fungicides to control diseases brings the concern about food and environmental safety. Screening new antimicrobial compounds and exploring involved mechanisms have great significance to development of new disease management strategies. Nitric oxide (NO), as an important intracellular signaling molecule, has been proved to be involved in many physiological processes and defense responses during plant pathogen interactions. In this study, we firstly found that NO at high concentration could distinctly delay spore germination and significantly reduce virulence of P. expansum to fruit host, identified some important proteins in response to NO stress and characterized the functions of these proteins. These results provide novel evidence for understanding the mechanism of NO regulating virulence of the fungal pathogen, but are beneficial for screening new targets of antifungal compounds. (c) 2014 Elsevier B.V. All rights reserved.
关键词	Penicillium expansum Nitric oxide Two dimension electrophoresis RT-PCR Energy metabolism
学科领域	Biochemical Research Methods
DOI	10.1016/j.jprot.2014.03.012
收录类别	SCI
语种	英语
WOS关键词	PLANT-PATHOGEN INTERACTIONS ; CRYPTOCOCCUS-LAURENTII ; BIOCONTROL ACTIVITY ; ASPERGILLUS-NIGER ; SPORE GERMINATION ; SALICYLIC-ACID ; JUJUBE FRUIT ; APPLE FRUIT ; STRESS ; DIOXYGENASE
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000337858700005
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	1876-7737
资助机构	National Natural Science Foundation of China [31030051, 31172005] ; Ministry of Science and Technology of the People's Republic of China [2012BAD38B05] ; National High Technology Research (863) Program of China [2012AA101607]
作者邮箱	tsp@ibcas.ac.cn
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27230
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Lai, Tongfei,Chen, Yong,Li, Boqiang,et al. Mechanism of Penicillium expansum in response to exogenous nitric oxide based on proteomics analysis[J]. JOURNAL OF PROTEOMICS,2014,103:47-56.
APA	Lai, Tongfei,Chen, Yong,Li, Boqiang,Qin, Guozheng,&Tian, Shiping.(2014).Mechanism of Penicillium expansum in response to exogenous nitric oxide based on proteomics analysis.JOURNAL OF PROTEOMICS,103,47-56.
MLA	Lai, Tongfei,et al."Mechanism of Penicillium expansum in response to exogenous nitric oxide based on proteomics analysis".JOURNAL OF PROTEOMICS 103(2014):47-56.

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