The evolutionary fate of Delta 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid Brassica napus

doi:10.1111/jse.12087

	The evolutionary fate of Delta 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid Brassica napus
	Wang, Cui-Ping ; Lin, Bin 1; Zhang, Yue-Qin ; Lin, Yue-Hui ; Liu, Ai-Hua ; Hua, Xue-Jun
	2014
发表期刊	JOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN	1674-4918
卷号	52 期号:5 页码:566-579
摘要	Although the regulatory and the functional aspects of proline accumulation in plant stress tolerance have been well-documented since it was discovered more than half a century ago, the evolution of proline metabolic genes in polyploidy species and their diploidy progenitors is not understood so far. Here, we have studied the retention and the expression regulation of 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid, Brassica napus, in comparison with its diploids progenitors, B. rapa and B. oleracea under salt stress. Our results indicated that all six P5CS1 genes from two diploid progenitors were retained. Sequence analysis revealed that all Brassica P5CS1 genes had a highly conserved exon-intron structure, similar to P5CS1 in Arabidopsis. In addition, two homeologous genes with different origin, BnaA.P5CS1.a and BnaC.P5CS1.d exhibited biased expression in different organs in B. napus, implying possible sub-functionalization of P5CS1 in B. napus. All six P5CS1 genes were induced in response to salt stress in B. napus, which rendered gene dose advantage for the level of proline accumulation. These results indicate that in B. napus, P5CS1 gene loss has not occurred during polyploidization, which suggest conserved functionality and evolutionary benefit of proline accumulation for plant adaptation to environmental stresses.
关键词	homeologous gene polyploidization proline accumulation salt stress sub-functionalization
学科领域	Plant Sciences
DOI	10.1111/jse.12087
收录类别	SCI
语种	英语
WOS关键词	ORNITHINE-DELTA-AMINOTRANSFERASE ; INCREASES PROLINE PRODUCTION ; FLOWERING-TIME-VARIATION ; DELTA(1)-PYRROLINE-5-CARBOXYLATE SYNTHETASE ; TRANSCRIPTIONAL REGULATION ; DIFFERENTIAL EXPRESSION ; FUNCTIONAL DIVERGENCE ; ARABIDOPSIS-THALIANA ; SALT TOLERANCE ; ACCUMULATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000342853300003
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1759-6831
资助机构	National Project for Transgenic Research from the Ministry of Agriculture of China [2011ZX08009-003] ; National Natural Science Foundation of China [30900091]
作者邮箱	liua@umanitoba.ca ; xjhua@ibcas.ac.cn
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27250
专题	中科院北方资源植物重点实验室
作者单位	1.[Wang, Cui-Ping 2.Lin, Bin 3.Zhang, Yue-Qin 4.Lin, Yue-Hui 5.Liu, Ai-Hua 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Univ Manitoba, Dept Plant Sci, Winnipeg, MB R3T 2N2, Canada
推荐引用方式 GB/T 7714	Wang, Cui-Ping,Lin, Bin,Zhang, Yue-Qin,et al. The evolutionary fate of Delta 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid Brassica napus[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2014,52(5):566-579.
APA	Wang, Cui-Ping,Lin, Bin,Zhang, Yue-Qin,Lin, Yue-Hui,Liu, Ai-Hua,&Hua, Xue-Jun.(2014).The evolutionary fate of Delta 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid Brassica napus.JOURNAL OF SYSTEMATICS AND EVOLUTION,52(5),566-579.
MLA	Wang, Cui-Ping,et al."The evolutionary fate of Delta 1-pyrroline-5-carboxylate synthetase 1 (P5CS1) genes in allotetraploid Brassica napus".JOURNAL OF SYSTEMATICS AND EVOLUTION 52.5(2014):566-579.

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