RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence

doi:10.1111/tpj.12494

	RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence
	Lu, Peitao ; Zhang, Changqing ; Liu, Jitao ; Liu, Xiaowei ; Jiang, Guimei ; Jiang, Xinqiang ; Khan, Muhammad Ali ; Wang, Liangsheng1 ; Hong, Bo ; Gao, Junping
	2014
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	78 期号:4 页码:578-590
摘要	Rose (Rosa hybrida) is one of the most important ornamental plants worldwide; however, senescence of its petals terminates the ornamental value of the flower, resulting in major economic loss. It is known that the hormones abscisic acid (ABA) and ethylene promote petal senescence, while gibberellins (GAs) delay the process. However, the molecular mechanisms underlying the antagonistic effects amongst plant hormones during petal senescence are still unclear. Here we isolated RhHB1, a homeodomain-leucine zipper I transcription factor gene, from rose flowers. Quantitative RT-PCR and GUS reporter analyses showed that RhHB1 was strongly expressed in senescing petals, and its expression was induced by ABA or ethylene in petals. ABA or ethylene treatment clearly accelerated rose petal senescence, while application of the gibberellin GA(3) delayed the process. However, silencing of RhHB1 delayed the ABA- or ethylene-mediated senescence, and resulted in higher petal anthocyanin levels and lower expression of RhSAG12. Moreover, treatment with paclobutrazol, an inhibitor of GA biosynthesis, repressed these delays. In addition, silencing of RhHB1 blocked the ABA- or ethylene-induced reduction in expression of the GA20 oxidase encoded by RhGA20ox1, a gene in the GA biosynthetic pathway. Furthermore, RhHB1 directly binds to the RhGA20ox1 promoter, and silencing of RhGA20ox1 promoted petal senescence. Eight senescence-related genes showed substantial differences in expression in petals after treatment with GA(3) or paclobutrazol. These results suggest that RhHB1 mediates the antagonistic effect of GAs on ABA and ethylene during rose petal senescence, and that the promotion of petal senescence by ABA or ethylene operates through an RhHB1-RhGA20ox1 regulatory checkpoint.
关键词	Rosa hybrida flower senescence RhHB1 gibberellins abscisic acid ethylene
学科领域	Plant Sciences
DOI	10.1111/tpj.12494
收录类别	SCI
语种	英语
WOS关键词	HD-ZIP PROTEIN ; ABSCISIC-ACID ; LEAF SENESCENCE ; GENE-EXPRESSION ; PERIANTH SENESCENCE ; SIGNALING PATHWAYS ; PETUNIA FLOWERS ; ARABIDOPSIS ; CARNATION ; HOMEODOMAIN
WOS研究方向	Plant Sciences
WOS记录号	WOS:000335537600004
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-313X
资助机构	National Natural Science Foundation of China [31130048, 31171992, 31071827]
作者邮箱	gaojp@cau.edu.cn
作品OA属性	Bronze
引用统计	被引频次：123[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27252
专题	中科院北方资源植物重点实验室
作者单位	1.China Agr Univ, Dept Ornamental Hort, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Lu, Peitao,Zhang, Changqing,Liu, Jitao,et al. RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence[J]. PLANT JOURNAL,2014,78(4):578-590.
APA	Lu, Peitao.,Zhang, Changqing.,Liu, Jitao.,Liu, Xiaowei.,Jiang, Guimei.,...&Gao, Junping.(2014).RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence.PLANT JOURNAL,78(4),578-590.
MLA	Lu, Peitao,et al."RhHB1 mediates the antagonism of gibberellins to ABA and ethylene during rose ( Rosa hybrida) petal senescence".PLANT JOURNAL 78.4(2014):578-590.

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