Knocking Out Bcsas1 in Botrytis cinerea Impacts Growth, Development, and Secretion of Extracellular Proteins, Which Decreases Virulence

doi:10.1094/MPMI-10-13-0314-R

	Knocking Out Bcsas1 in Botrytis cinerea Impacts Growth, Development, and Secretion of Extracellular Proteins, Which Decreases Virulence
	Zhang, Zhanquan; Qin, Guozheng; Li, Boqiang; Tian, Shiping
	2014
发表期刊	MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN	0894-0282
卷号	27 期号:6 页码:590-600
摘要	Pathogenic fungi usually secrete a series of virulence factors to the extracellular environment to facilitate infection. Rab GTPases play a central role in the secretory pathway. To explore the function of Rab/GTPase in filamentous fungi, we knocked out a Rab/GTPase family gene, Bcsas1, in Botrytis cinerea, an aggressive fungal pathogen that infects more than 200 plant species. A detailed analysis was conducted on the virulence and the secretory capability of the mutants. The results indicated that knockout of Bcsas1 inhibited hyphal development and reduced sporulation of B. cinerea on potato dextrose agar plates resulting in reduced virulence on various fruit hosts. Knocking out the Bcsas1 gene led to an accumulation of transport vesicles at the hyphal tip, significantly reduced extracellular protein content, and lowered the activity of polygalacturonase and xylanase in the extracellular medium. However, mutation of Bcsas1 did not affect the expression of genes encoding polygalacturonase and xylanase, suggesting the secretion of these two family enzymes was suppressed in the mutant. Moreover, a comparative analysis of the secretome provided further evidence that the disruption of Bcsas1 in mutant strains significantly depressed the secretion of polysaccharide hydrolases and proteases. The results indicate that Bcsas1, the Rab8/SEC4-like gene, plays a crucial role in development, protein secretion, and virulence of B. cinerea.
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.1094/MPMI-10-13-0314-R
收录类别	SCI
语种	英语
WOS关键词	MOLECULAR CHARACTERIZATION ; FILAMENTOUS FUNGI ; RAB FAMILY ; MEMBRANE ; GENE ; BETA-1,3-EXOGLUCANASE ; CONTRIBUTES ; SPECIFICITY ; ENDOCYTOSIS ; TRAFFICKING
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:000335635500009
出版者	AMER PHYTOPATHOLOGICAL SOC
文献子类	Article
出版地	ST PAUL
EISSN	1943-7706
资助机构	973 program [2013CB127103] ; National Natural Science Foundation of China [31030051] ; Chinese Academy of Sciences [KSCX2-EW-G-6]
作者邮箱	tsp@ibcas.ac.cn
作品OA属性	hybrid
引用统计	被引频次：57[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27254
专题	中科院北方资源植物重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zhanquan,Qin, Guozheng,Li, Boqiang,et al. Knocking Out Bcsas1 in Botrytis cinerea Impacts Growth, Development, and Secretion of Extracellular Proteins, Which Decreases Virulence[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2014,27(6):590-600.
APA	Zhang, Zhanquan,Qin, Guozheng,Li, Boqiang,&Tian, Shiping.(2014).Knocking Out Bcsas1 in Botrytis cinerea Impacts Growth, Development, and Secretion of Extracellular Proteins, Which Decreases Virulence.MOLECULAR PLANT-MICROBE INTERACTIONS,27(6),590-600.
MLA	Zhang, Zhanquan,et al."Knocking Out Bcsas1 in Botrytis cinerea Impacts Growth, Development, and Secretion of Extracellular Proteins, Which Decreases Virulence".MOLECULAR PLANT-MICROBE INTERACTIONS 27.6(2014):590-600.

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