Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system

doi:10.1093/jxb/eru351

	Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system
	Chen, Xianyang; Bao, Hexigeduleng; Guo, Jie; Jia, Weitao; Tai, Fang; Nie, Lingling; Jiang, Ping; Feng, Juanjuan; Lv, Sulian; Li, Yinxin
	2014
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	65 期号:20 页码:6107-6122
摘要	Despite the importance of NHX1 (Na+/H+ exchanger 1) in plant salt tolerance, little is known about its other functions. In this study, intriguingly, it was found that NHX1 participated in plant disease defence against Phytophthora parasitica var. nicotianae (Ppn) in Nicotiana benthamiana. NbNHX1 was originally isolated from N. benthamiana, and characterized. The subcellular localization of NbNHX1 with its C-terminus fused with green fluorescent protein indicated that NbNHX1 localized primarily to the tonoplast. Tobacco rattle virus-induced NbNHX1 silencing led to reduced H+ efflux from the vacuole to cytoplasts, and decreased Ppn resistance in N. benthamiana. After attack by Ppn, NbNHX1-silenced plants exhibited impaired ability to scavenge reactive oxidative species (ROS) induced by the pathogen. Pea early browning virus-mediated ectopic expression of SeNHX1 (from Salicornia europaea) or AtNHX1 (from Arabidopsis thaliana) both conferred enhanced Ppn resistance to N. benthamiana, with a lower H2O2 concentration after Ppn inoculation. Further investigation of the role of NHX1 demonstrated that transient overexpression of NbNHX1 improved the vacuolar pH and cellular ROS level in N. benthamiana, which was coupled with an enlarged NAD(P) (H) pool and higher expression of ROS-responsive genes. In contrast, NbNHX1 silencing led to a lower pH in the vacuole and a lower cellular ROS level in N. benthamiana, which was coupled with a decreased NAD(P) (H) pool and decreased expression of ROS-responsive genes. These results suggest that NHX1 is involved in plant disease defence; and regulation of vacuolar pH by NHX1, affecting the cellular oxidation state, primes the antioxidative system which is associated with Ppn resistance in tobacco.
关键词	Cellular redox homeostasis disease resistance NAD(P) (H) pool NHX1 tobacco vacuolar H+ flux and pH
学科领域	Plant Sciences
DOI	10.1093/jxb/eru351
收录类别	SCI
语种	英语
WOS关键词	CONFERS SALT-TOLERANCE ; REACTIVE OXYGEN ; ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; GENE-EXPRESSION ; ANTIPORTER GENE ; CELL-DEATH ; HOMEOSTASIS ; OVEREXPRESSION ; PLANTS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000344658800032
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	Chinese Ministry of Agriculture [2013ZX08009-003-002] ; National Natural Science Foundation of China [31200201]
作者邮箱	yxli@ibcas.ac.cn
作品OA属性	Green Submitted, hybrid, Green Published
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27256
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xianyang,Bao, Hexigeduleng,Guo, Jie,et al. Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(20):6107-6122.
APA	Chen, Xianyang.,Bao, Hexigeduleng.,Guo, Jie.,Jia, Weitao.,Tai, Fang.,...&Li, Yinxin.(2014).Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system.JOURNAL OF EXPERIMENTAL BOTANY,65(20),6107-6122.
MLA	Chen, Xianyang,et al."Na+/H+ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system".JOURNAL OF EXPERIMENTAL BOTANY 65.20(2014):6107-6122.

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