Effects of silver nitrate on shoot regeneration of Artemisia annua L.

doi:10.5511/plantbiotechnology.13.1109a

	Effects of silver nitrate on shoot regeneration of Artemisia annua L.
	Lei, Caiyan 1,2; Wang, Hong 2; Liu, Benye 1; Ye, Hechun 1
	2014
发表期刊	PLANT BIOTECHNOLOGY
ISSN	1342-4580
卷号	31 期号:1 页码:71-75
摘要	In order to further increase the shoot regeneration frequency of Artemisia annua L., the effects of silver nitrate on this process was investigated in this study. Different concentration of silver nitrate was added to the shoot induction medium, which was MS basic medium containing 1.0 mgl(-1) 6-benzyladenine (6-BA) and 0.05 mgl(-1) alpha-napthaleneacetic acid (NAA). When 2 mgl(-1) silver nitrate was added to the shoot induction medium, the shoot induction frequency and shoot number per explants was significantly higher than that of the control (without silver nitrate). In addition, silver nitrate at all the tested concentrations could significantly reduce callus formation of the explants. Silver nitrate had also positive influence on shoot elongation in the first 20 days. Furthermore, silver nitrate did not affect the sensitivity of A. annua shoots to Kanamycin (KM); therefore, silver nitrate could be used to improve shoot regeneration capacity and frequency in A. annua genetic transformation.
关键词	Artemisia annua L. elongation growth shoot induction silver nitrate
学科领域	Biotechnology & Applied Microbiology ; Plant Sciences
DOI	10.5511/plantbiotechnology.13.1109a
收录类别	SCI
语种	英语
WOS关键词	TUMEFACIENS-MEDIATED TRANSFORMATION ; PLANT-REGENERATION ; BIOSYNTHESIS ; IMPROVEMENT ; CULTIVARS ; ETHYLENE ; GROWTH
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:000332467700011
出版者	JAPANESE SOC PLANT CELL & MOLECULAR BIOLOGY
文献子类	Article
出版地	NARA
资助机构	Public Welfare (agriculture) Research Project China [201303030] ; Science and Technology Research Projects of Henan Province, China [13B210049]
作者邮箱	benyeliu@126.com
作品OA属性	Bronze
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27273
专题	中科院植物分子生理学重点实验室
作者单位	1.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lei, Caiyan,Wang, Hong,Liu, Benye,et al. Effects of silver nitrate on shoot regeneration of Artemisia annua L.[J]. PLANT BIOTECHNOLOGY,2014,31(1):71-75.
APA	Lei, Caiyan,Wang, Hong,Liu, Benye,&Ye, Hechun.(2014).Effects of silver nitrate on shoot regeneration of Artemisia annua L..PLANT BIOTECHNOLOGY,31(1),71-75.
MLA	Lei, Caiyan,et al."Effects of silver nitrate on shoot regeneration of Artemisia annua L.".PLANT BIOTECHNOLOGY 31.1(2014):71-75.

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