Deciphering the Physalis floridana Double-Layered-Lantern1 Mutant Provides Insights into Functional Divergence of the GLOBOSA Duplicates within the Solanaceae

doi:10.1104/pp.113.233072

	Deciphering the Physalis floridana Double-Layered-Lantern1 Mutant Provides Insights into Functional Divergence of the GLOBOSA Duplicates within the Solanaceae
	Zhang, Ji-Si ; Li, Zhichao ; Zhao, Jing ; Zhang, Shaohua ; Quan, Hui ; Zhao, Man 5; He, Chaoying6
	2014
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	164 期号:2 页码:748-764
摘要	Physalis spp. develop the Chinese lantern trait, also known as inflated calyx syndrome, that is a morphological novelty. Here, we identified the double-layered-lantern1 (doll1) mutant, a recessive and monofactorial mutation, in Physalis floridana; its corolla and androecium were transformed into the calyx and gynoecium, respectively. Two GLOBOSA-like MADS-box paralogous genes PFGLO1 and PFGLO2 were found in Physalis floridana, while the mutated phenotype was cosegregated with a large deletion harboring PFGLO1 and was complemented by the PFGLO1 genomic locus in transgenic plants, and severe PFGLO1 knockdowns phenocopied doll1. Thus, DOLL1 encodes the PFGLO1 protein and plays a primary role in determining corolla and androecium identity. However, specific PFGLO2 silencing showed no homeotic variation but rather affected pollen maturation. The two genes featured identical floral expression domains, but the encoding proteins shared 67% identity in sequences. PFGLO1 was localized in the nucleus when expressed in combination with a DEFICIENS homolog from Physalis floridana, whereas PFGLO2 was imported to the nucleus on its own. The two proteins were further found to have evolved different interacting partners and regulatory patterns, supporting the hypothesis that PFGLO2 is functionally separated from organ identity. Such a divergent pattern of duplicated GLO genes is unusual within the Solanaceae. Moreover, the phenotypes of the PFGLO1PFGLO2 double silencing mutants suggested that PFGLO2, through genetically interacting with PFGLO1, also exerts a role in the control of organ number and tip development of the second floral whorl. Our results, therefore, shed new light on the functional evolution of the duplicated GLO genes.
学科领域	Plant Sciences
DOI	10.1104/pp.113.233072
收录类别	SCI
语种	英语
WOS关键词	INFLATED CALYX SYNDROME ; MADS-BOX ; HOMEOTIC GENE ; HETEROTOPIC EXPRESSION ; MORPHOLOGICAL NOVELTY ; FLOWER DEVELOPMENT ; CHINESE LANTERN ; MOLECULAR-BASIS ; EVOLUTION ; DEFICIENS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000331132300020
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	1532-2548
资助机构	National Natural Science Foundation of China [31070203, 30870175] ; Hundred Talent Program of the Chinese Academy of Sciences ; Education Ministry of China
作者邮箱	chaoying@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27279
专题	系统与进化植物学国家重点实验室
作者单位	1.[Zhang, Ji-Si 2.Li, Zhichao 3.Zhao, Jing 4.Zhang, Shaohua 5.Quan, Hui 6.Zhao, Man 7.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing 100093, Peoples R China 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ji-Si,Li, Zhichao,Zhao, Jing,et al. Deciphering the Physalis floridana Double-Layered-Lantern1 Mutant Provides Insights into Functional Divergence of the GLOBOSA Duplicates within the Solanaceae[J]. PLANT PHYSIOLOGY,2014,164(2):748-764.
APA	Zhang, Ji-Si.,Li, Zhichao.,Zhao, Jing.,Zhang, Shaohua.,Quan, Hui.,...&He, Chaoying.(2014).Deciphering the Physalis floridana Double-Layered-Lantern1 Mutant Provides Insights into Functional Divergence of the GLOBOSA Duplicates within the Solanaceae.PLANT PHYSIOLOGY,164(2),748-764.
MLA	Zhang, Ji-Si,et al."Deciphering the Physalis floridana Double-Layered-Lantern1 Mutant Provides Insights into Functional Divergence of the GLOBOSA Duplicates within the Solanaceae".PLANT PHYSIOLOGY 164.2(2014):748-764.

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