Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes

doi:10.3967/bes2014.115

IB-CAS > 植物园

	Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes
	Xie Lei ; Wang Ying Wei 2; Guan Shan Yue 3; Xie Li Jing 1; Long Xin 1; Sun Cheng Ye 1
	2014
发表期刊	BIOMEDICAL AND ENVIRONMENTAL SCIENCES
ISSN	0895-3988
卷号	27 期号:10 页码:794-806
摘要	Objective Poisonous plants are a deadly threat to public health in China. The traditional clinical diagnosis of the toxic plants is inefficient, fallible, and dependent upon experts. In this study, we tested the performance of DNA barcodes for identification of the most threatening poisonous plants in China. Methods Seventy-four accessions of 27 toxic plant species in 22 genera and 17 families were sampled and three DNA barcodes (matK, rbcL, and ITS) were amplified, sequenced and tested. Three methods, Blast, pairwise global alignment (PWG) distance, and Tree-Building were tested for discrimination power. Results The primer universality of all the three markers was high. Except in the case of ITS for Hemerocallis minor, the three barcodes were successfully generated from all the selected species. Among the three methods applied, Blast showed the lowest discrimination rate, whereas PWG Distance and Tree-Building methods were equally effective. The ITS barcode showed highest discrimination rates using the PWG Distance and Tree-Building methods. When the barcodes were combined, discrimination rates were increased for the Blast method. Conclusion DNA barcoding technique provides us a fast tool for clinical identification of poisonous plants in China. We suggest matK, rbcL, ITS used in combination as DNA barcodes for authentication of poisonous plants.
关键词	Poisonous plants DNA barcoding matK rbcL ITS
学科领域	Environmental Sciences ; Public, Environmental & Occupational Health
DOI	10.3967/bes2014.115
收录类别	SCI
语种	英语
WOS关键词	SPECIES IDENTIFICATION ; LAND PLANTS ; RANUNCULACEAE ; IDENTIFY ; ACONITUM ; DELPHINIUM ; PHYLOGENY ; UTILITY
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS记录号	WOS:000344175900006
出版者	CHINESE CENTER DISEASE CONTROL & PREVENTION
文献子类	Article
出版地	BEIJING
EISSN	2214-0190
资助机构	Fundamental Research Funds for the Central Universities [TD2012-04] ; Scientific Research Foundation of the State Human Resource Ministry ; Education Ministry for Returned Chinese Scholars ; State Key Laboratory of Systematic and Evolutionary Botany [LSEB2011-07] ; Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences [KSCX2-EW-Z-1] ; National Natural Science Foundation of China [31170201, 81072317, 31110103911]
作者邮箱	pccsun@gmail.com
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27285
专题	植物园
作者单位	1.Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China 2.Natl Inst Occupat Hlth & Poison Control, China Ctr Dis Control & Prevent, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China 4.Clemson Univ, Sch Agr Forestry & Environm Sci Forest Resources, Clemson, SC 29634 USA
推荐引用方式 GB/T 7714	Xie Lei,Wang Ying Wei,Guan Shan Yue,et al. Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2014,27(10):794-806.
APA	Xie Lei,Wang Ying Wei,Guan Shan Yue,Xie Li Jing,Long Xin,&Sun Cheng Ye.(2014).Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,27(10),794-806.
MLA	Xie Lei,et al."Prospects and Problems for Identification of Poisonous Plants in China using DNA Barcodes".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 27.10(2014):794-806.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
25341815.pdf（5058KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文