Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment

doi:10.1007/s10529-013-1418-9

IB-CAS > 中科院光生物学重点实验室

	Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment
	Tian, Qi-Lin ; Shi, Ding-Ji ; Jia, Xiao-Hui ; Mi, Hua-Ling ; Huang, Xi-Wen ; He, Pei-Min
	2014
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
卷号	36 期号:4 页码:821-827
摘要	To investigate the function of a bacterial-type phosphoenolpyruvate carboxylase (PEPC2) derived from photosynthetically-grown Chlamydomonas reinhardtii, a fragment of the pepc2 gene was cloned and expressed in Escherichia coli. After optimal induction for 6 h, PEPC activity in the reverse mutant was lower than wild type (0.9 vs. 1.7 U/mg protein), and soluble protein was also lower than wild type (119 vs. 186 mg/g dry wt). In contrast, the total lipid content was increased from 56 (in wild type) to 71 mg/g dry wt, despite the growth rate being slightly diminished. The changes in PEPC activity, soluble protein and total lipid in the forward mutant were the opposite (2.4 U/mg, 230 mg/g, and 44 mg/g dry wt, respectively). Together, these data indicate that PEPC may function as a metabolic pivot in the regulation of protein and lipid accumulation in this alga.
关键词	Algal lipids Chlamydomonas reinhardtii Escherichia coli Gene expression Lipid production Phosphoenolpyruvate carboxylase Reverse genetics
学科领域	Biotechnology & Applied Microbiology
DOI	10.1007/s10529-013-1418-9
收录类别	SCI
语种	英语
WOS关键词	IDENTIFICATION ; EVOLUTION
WOS研究方向	Biotechnology & Applied Microbiology
WOS记录号	WOS:000333110500019
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-6776
资助机构	National High Technology Research and Development Program of China (863 Program) [2009AA064401] ; Shanghai Universities First-Class Disciplines Project [0707]
作者邮箱	waichifanqie@126.com ; cyano.shi@yahoo.com.cn ; xhjia@shou.edu.cn ; mihl@sippe.ac.cn ; hu0710092@126.com ; pmhe@shou.edu.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27292
专题	中科院光生物学重点实验室
作者单位	1.[Tian, Qi-Lin 2.Shi, Ding-Ji 3.Jia, Xiao-Hui 4.Huang, Xi-Wen 5.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China 6.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 7.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Tian, Qi-Lin,Shi, Ding-Ji,Jia, Xiao-Hui,et al. Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment[J]. BIOTECHNOLOGY LETTERS,2014,36(4):821-827.
APA	Tian, Qi-Lin,Shi, Ding-Ji,Jia, Xiao-Hui,Mi, Hua-Ling,Huang, Xi-Wen,&He, Pei-Min.(2014).Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment.BIOTECHNOLOGY LETTERS,36(4),821-827.
MLA	Tian, Qi-Lin,et al."Recombinant expression and functional analysis of a Chlamydomonas reinhardtii bacterial-type phosphoenolpyruvate carboxylase gene fragment".BIOTECHNOLOGY LETTERS 36.4(2014):821-827.

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