Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness

doi:10.1371/journal.pone.0101905

	Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness
	Meng, Hongyan ; Jiang, Ling 1; Xu, Bosi ; Guo, Wenzhu 2; Li, Jinglai 3; Zhu, Xiuqing 3; Qi, Xiaoquan4 ; Duan, Lixin 4; Meng, Xianbin 4; Fan, Yunliu 1; Zhang, Chunyi 1
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:7
摘要	Interactions among metabolic pathways are important in plant biology. At present, not much is known about how folate metabolism affects other metabolic pathways in plants. Here we report a T-DNA insertion mutant (atdfb-3) of the plastidial folylpolyglutamate synthetase gene (AtDFB) was defective in seed reserves and skotomorphogenesis. Lower carbon (C) and higher nitrogen (N) content in the mutant seeds than that of the wild type were indicative of an altered C and N partitioning capacity. Higher levels of organic acids and sugars were detected in the mutant seeds compared with the wild type. Further analysis revealed that atdfb-3 seeds contained less total amino acids and individual Asn and Glu as well as NO3-. These results indicate significant changes in seed storage in the mutant. Defects in hypocotyl elongation were observed in atdfb-3 in darkness under sufficient NO3- conditions, and further enhanced under NO3- limited conditions. The strong expression of AtDFB in cotyledons and hypocotyl during early developmental stage was consistent with the mutant sensitivity to limited NO3- during a narrow developmental window. Exogenous 5-formyl-tetrahydrofolate completely restored the hypocotyl length in atdfb-3 seedlings with NO3- as the sole N source. Further study demonstrated that folate profiling and N metabolism were perturbed in atdfb-3 etiolated seedlings. The activity of enzymes involved in N reduction and assimilation was altered in atdfb-3. Taken together, these results indicate that AtDFB is required for seed reserves, hypocotyl elongation and N metabolism in darkness, providing novel insights into potential associations of folate metabolism with seed reserve accumulation, N metabolism and hypocotyl development in Arabidopsis.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0101905
收录类别	SCI
语种	英语
WOS关键词	ONE-CARBON METABOLISM ; PISUM-SATIVUM-L ; HIGHER-PLANTS ; TETRAHYDROFOLATE BIOSYNTHESIS ; FOLATE POLYGLUTAMYLATION ; CELL ELONGATION ; GERMINATION ; THALIANA ; GROWTH ; ACID
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000338637300100
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Basic Research Program of China [2013CB127003]
作者邮箱	zhangchunyi@caas.cn
作品OA属性	Green Published, Green Submitted, gold
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27315
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China 2.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China 3.Huazhong Agr Univ, Wuhan, Peoples R China 4.Beijing Inst Pharmacol & Toxicol, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Meng, Hongyan,Jiang, Ling,Xu, Bosi,et al. Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness[J]. PLOS ONE,2014,9(7).
APA	Meng, Hongyan.,Jiang, Ling.,Xu, Bosi.,Guo, Wenzhu.,Li, Jinglai.,...&Zhang, Chunyi.(2014).Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness.PLOS ONE,9(7).
MLA	Meng, Hongyan,et al."Arabidopsis Plastidial Folylpolyglutamate Synthetase Is Required for Seed Reserve Accumulation and Seedling Establishment in Darkness".PLOS ONE 9.7(2014).

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