FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis

doi:10.1371/journal.pone.0099741

	FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis
	Lu, Xiaoduo ; Zhang, Dongyuan 1; Li, Shipeng ; Su, Yanping 2; Liang, Qiuju 3,4; Meng, Hongyan 3; Shen, Songdong 2; Fan, Yunliu 3,4; Liu, Chunming5 ; Zhang, Chunyi 3,4
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:6
摘要	Chloroplast formation is associated with embryo development and seedling growth. However, the relationship between chloroplast differentiation and embryo development remains unclear. Five FtsHi genes that encode proteins with high similarity to FtsH proteins, but lack Zn2+-binding motifs, are present in the Arabidopsis genome. In this study, we showed that T-DNA insertion mutations in the Arabidopsis FtsHi4 gene resulted in embryo arrest at the globular-to-heart-shaped transition stage. Transmission electron microscopic analyses revealed abnormal plastid differentiation with a severe defect in thylakoid formation in the mutant embryos. Immunocytological studies demonstrated that FtsHi4 localized in chloroplasts as a thylakoid membrane-associated protein, supporting its essential role in thylakoid membrane formation. We further showed that FtsHi4 forms protein complexes, and that there was a significant reduction in the accumulation of D2 and PsbO (two photosystem II proteins) in mutant ovules. The role of FtsHi4 in chloroplast development was confirmed using an RNA-interfering approach. Additionally, mutations in other FtsHi genes including FtsHi1, FtsHi2, and FtsHi5 caused phenotypic abnormalities similar to ftshi4 with respect to plastid differentiation during embryogenesis. Taken together, our data suggest that FtsHi4, together with FtsHi1, FtsHi2, and FtsHi5 are essential for chloroplast development in Arabidopsis.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0099741
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYSTEM-II REPAIR ; THYLAKOID MEMBRANE ; EMBRYO DEVELOPMENT ; GENE-EXPRESSION ; CHLOROPHYLL FLUORESCENCE ; PLANT DEVELOPMENT ; LEAF VARIEGATION ; SEED DEVELOPMENT ; PROTEIN IMPORT ; D1 PROTEIN
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000338709500017
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Science Foundation of China [31071077]
作者邮箱	zhangchunyi@caas.cn
作品OA属性	Green Published, gold
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27318
专题	中科院植物分子生理学重点实验室
作者单位	1.Qilu Normal Univ, Dept Life Sci, Jinan, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 3.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou, Peoples R China 4.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100193, Peoples R China 5.Natl Key Facil Crop Gene Resources & Genet Improv, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Lu, Xiaoduo,Zhang, Dongyuan,Li, Shipeng,et al. FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis[J]. PLOS ONE,2014,9(6).
APA	Lu, Xiaoduo.,Zhang, Dongyuan.,Li, Shipeng.,Su, Yanping.,Liang, Qiuju.,...&Zhang, Chunyi.(2014).FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis.PLOS ONE,9(6).
MLA	Lu, Xiaoduo,et al."FtsHi4 Is Essential for Embryogenesis Due to Its Influence on Chloroplast Development in Arabidopsis".PLOS ONE 9.6(2014).

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