Slow Co-Evolution of the MAGO and Y14 Protein Families Is Required for the Maintenance of Their Obligate Heterodimerization Mode

doi:10.1371/journal.pone.0084842

	Slow Co-Evolution of the MAGO and Y14 Protein Families Is Required for the Maintenance of Their Obligate Heterodimerization Mode
	Gong, Pichang 1; Zhao, Man 1; He, Chaoying
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:1
摘要	The exon junction complex (EJC) plays important roles in RNA metabolisms and the development of eukaryotic organisms. MAGO (short form of MAGO NASHI) and Y14 (also Tsunagi or RBM8) are the EJC core components. Their biological roles have been well investigated in various species, but the evolutionary patterns of the two gene families and their protein-protein interactions are poorly known. Genome-wide survey suggested that the MAGO and Y14 two gene families originated in eukaryotic organisms with the maintenance of a low copy. We found that the two protein families evolved slowly; however, the MAGO family under stringent purifying selection evolved more slowly than the Y14 family that was under relative relaxed purifying selection. MAGO and Y14 were obliged to form heterodimer in a eukaryotic organism, and this obligate mode was plesiomorphic. Lack of binding of MAGO to Y14 as functional barrier was observed only among distantly species, suggesting that a slow co-evolution of the two protein families. Inter-protein co-evolutionary signal was further quantified in analyses of the Tol-MirroTree and co-evolution analysis using protein sequences. About 20% of the 41 significantly correlated mutation groups (involving 97 residues) predicted between the two families was clade-specific. Moreover, around half of the predicted co-evolved groups and nearly all clade-specific residues fell into the minimal interaction domains of the two protein families. The mutagenesis effects of the clade-specific residues strengthened that the co-evolution is required for obligate MAGO-Y14 heterodimerization mode. In turn, the obliged heterodimerization in an organism serves as a strong functional constraint for the co-evolution of the MAGO and Y14 families. Such a co-evolution allows maintaining the interaction between the proteins through large evolutionary time scales. Our work shed a light on functional evolution of the EJC genes in eukaryotes, and facilitates to understand the co-evolutionary processes among protein families.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0084842
收录类别	SCI
语种	英语
WOS关键词	EXON JUNCTION COMPLEX ; OSKAR MESSENGER-RNA ; EVOLUTIONARY RATE ; BINDING PROTEIN ; NASHI ; GENE ; OOCYTE ; INTERACTS ; SUBSTITUTION ; DIFFERENTIATION
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000329862500179
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Chinese Ministry of Agriculture Transgenic Major Project [2009ZX08009-011B] ; Chinese Academy of Sciences
作者邮箱	chaoying@ibcas.ac.cn
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27338
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gong, Pichang,Zhao, Man,He, Chaoying. Slow Co-Evolution of the MAGO and Y14 Protein Families Is Required for the Maintenance of Their Obligate Heterodimerization Mode[J]. PLOS ONE,2014,9(1).
APA	Gong, Pichang,Zhao, Man,&He, Chaoying.(2014).Slow Co-Evolution of the MAGO and Y14 Protein Families Is Required for the Maintenance of Their Obligate Heterodimerization Mode.PLOS ONE,9(1).
MLA	Gong, Pichang,et al."Slow Co-Evolution of the MAGO and Y14 Protein Families Is Required for the Maintenance of Their Obligate Heterodimerization Mode".PLOS ONE 9.1(2014).

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