Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation

doi:10.1371/journal.pone.0113772

	Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation
	Xi, Huifen 1,2; Ma, Ling 1,2; Liu, Guotian 1,2; Wang, Nian ; Wang, Junfang 1,2; Wang, Lina 1,3; Dai, Zhanwu4 ; Li, Shaohua1,3 ; Wang, Lijun 1
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:12
摘要	Background: Only a small amount of solar ultraviolet C (UV-C) radiation reaches the Earth's surface. This is because of the filtering effects of the stratospheric ozone layer. Artificial UV-C irradiation is used on leaves and fruits to stimulate different biological processes in plants. Grapes are a major fruit crop and are grown in many parts of the world. Research has shown that UV-C irradiation induces the biosynthesis of phenols in grape leaves. However, few studies have analyzed the overall changes in gene expression in grape leaves exposed to UV-C. Methodology/Principal Findings: In the present study, transcriptional responses were investigated in grape (Vitis vinifera L.) leaves before and after exposure to UV-C irradiation (6 W.m(-2) for 10 min) using an Affymetrix Vitis vinifera (Grape) Genome Array (15,700 transcripts). A total of 5274 differentially expressed probe sets were defined, including 3564 (67.58%) probe sets that appeared at both 6 and 12 h after exposure to UV-C irradiation but not before exposure. A total of 468 (8.87%) probe sets and 1242 (23.55%) probe sets were specifically expressed at these times. The probe sets were associated with a large number of important traits and biological pathways, including cell rescue (i.e., antioxidant enzymes), protein fate (i.e., HSPs), primary and secondary metabolism, and transcription factors. Interestingly, some of the genes involved in secondary metabolism, such as stilbene synthase, responded intensely to irradiation. Some of the MYB and WRKY family transcription factors, such as VvMYBPA1, VvMYB14, VvMYB4, WRKY57-like, and WRKY 65, were also strongly up-regulated (about 100 to 200 fold). Conclusions: UV-C irridiation has an important role in some biology processes, especially cell rescue, protein fate, secondary metabolism, and regulation of transcription. These results opened up ways of exploring the molecular mechanisms underlying the effects of UV-C irradiation on grape leaves and have great implications for further studies.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0113772
收录类别	SCI
语种	英语
WOS关键词	UV-C ; CABERNET-SAUVIGNON ; ANTIOXIDANT ENZYMES ; STRESS RESPONSES ; RADIATION ; BIOSYNTHESIS ; PLANTS ; RESVERATROL ; GENES ; ACCUMULATION
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000345869700052
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China [31171918]
作者邮箱	Shhli@ibcas.ac.cn ; ljwang@ibcas.ac.cn
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27362
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 5.INRA, ISVV, UMR EGFV 1287, Villenave Dornon, France
推荐引用方式 GB/T 7714	Xi, Huifen,Ma, Ling,Liu, Guotian,et al. Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation[J]. PLOS ONE,2014,9(12).
APA	Xi, Huifen.,Ma, Ling.,Liu, Guotian.,Wang, Nian.,Wang, Junfang.,...&Wang, Lijun.(2014).Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation.PLOS ONE,9(12).
MLA	Xi, Huifen,et al."Transcriptomic Analysis of Grape (Vitis vinifera L.) Leaves after Exposure to Ultraviolet C Irradiation".PLOS ONE 9.12(2014).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Xi-2014-Transcriptom（1229KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文