Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera

doi:10.1186/1471-2229-14-103

	Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera
	Wang, Lina 2; Zhu, Wei 2; Fang, Linchuan 2; Sun, Xiaoming 2; Su, Lingye 1,2; Liang, Zhenchang 1,2; Wang, Nian 1; Londo, Jason P.; Li, Shaohua1 ; Xin, Haiping 1
	2014
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	14
摘要	Background: WRKY transcription factors are one of the largest families of transcriptional regulators in plants. WRKY genes are not only found to play significant roles in biotic and abiotic stress response, but also regulate growth and development. Grapevine (Vitis vinifera) production is largely limited by stressful climate conditions such as cold stress and the role of WRKY genes in the survival of grapevine under these conditions remains unknown. Results: We identified a total of 59 VvWRKYs from the V. vinifera genome, belonging to four subgroups according to conserved WRKY domains and zinc-finger structure. The majority of VvWRKYs were expressed in more than one tissue among the 7 tissues examined which included young leaves, mature leaves, tendril, stem apex, root, young fruits and ripe fruits. Publicly available microarray data suggested that a subset of VvWRKYs was activated in response to diverse stresses. Quantitative real-time PCR (qRT-PCR) results demonstrated that the expression levels of 36 VvWRKYs are changed following cold exposure. Comparative analysis was performed on data from publicly available microarray experiments, previous global transcriptome analysis studies, and qRT-PCR. We identified 15 VvWRKYs in at least two of these databases which may relate to cold stress. Among them, the transcription of three genes can be induced by exogenous ABA application, suggesting that they can be involved in an ABA-dependent signaling pathway in response to cold stress. Conclusions: We identified 59 VvWRKYs from the V. vinifera genome and 15 of them showed cold stress-induced expression patterns. These genes represented candidate genes for future functional analysis of VvWRKYs involved in the low temperature-related signal pathways in grape.
关键词	WRKY transcription factor family Grapevine Biotic and abiotic stress Cold stress
学科领域	Plant Sciences
DOI	10.1186/1471-2229-14-103
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR FAMILY ; DNA-BINDING PROTEINS ; DROUGHT TOLERANCE ; ABSCISIC-ACID ; CHITINASE GENE ; ARABIDOPSIS ; EXPRESSION ; GRAPEVINE ; RESISTANCE ; RICE
WOS研究方向	Plant Sciences
WOS记录号	WOS:000335344600001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China (NSFC) [31130047, 31000902]
作者邮箱	shhli@wbgcas.cn ; xinhaiping215@hotmail.com
作品OA属性	Green Published, gold
引用统计	被引频次：154[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27368
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Londo, Jason P.] USDA ARS, Grape Genet Res Unit, Geneva, NY USA
推荐引用方式 GB/T 7714	Wang, Lina,Zhu, Wei,Fang, Linchuan,et al. Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera[J]. BMC PLANT BIOLOGY,2014,14.
APA	Wang, Lina.,Zhu, Wei.,Fang, Linchuan.,Sun, Xiaoming.,Su, Lingye.,...&Xin, Haiping.(2014).Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera.BMC PLANT BIOLOGY,14.
MLA	Wang, Lina,et al."Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera".BMC PLANT BIOLOGY 14(2014).

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