Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding

doi:10.1371/journal.pone.0089495

	Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding
	Chen, Shuangyan ; Cai, Yueyue 1; Zhang, Lexin 2; Yan, Xueqing 1; Cheng, Liqin ; Qi, Dongmei ; Zhou, Qingyuan ; Li, Xiaoxia ; Liu, Gongshe
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:2
摘要	Background: Herbivore grazing is a multiple-component process that includes wounding, defoliation, and saliva deposition. Despite the extensive published research on mechanical wounding and defoliation, no analysis to identify the genes that specify defoliation and mechanical wounding has been performed. Moreover, the influence of the expression of these genes on plant regrowth after defoliation remains poorly understood. Results: Seven cDNA libraries for RNA samples collected from stubble tissues that had been mechanically wounded or defoliated at 2, 6 and 24 h along with the control were sequenced using the Illumina/Solexa platform. A comparative transcriptomic analysis of the sequencing data was conducted. In total, 1,836 and 3,238 genes were detected with significant differential expression levels after wounding and defoliation, respectively, during one day. GO, KOG and pathway-based enrichment analyses were performed to determine and further understand the biological functions of those differentially expressed genes (DEGs). The results demonstrated that both wounding and defoliation activated the systemic synthesis of jasmonate (JA). However, defoliation specifically reduced the expression levels of ribosomal protein genes, cell division or cell expansion-related genes, and lignin biosynthesis genes and may have negatively affected plant growth. Further analysis revealed that the regrowth of elongating leaves was significantly retarded after defoliation at 6 h through the following 7 days of measurement, suggesting that the gene expression pattern and phenotype are consistent. Fifteen genes were selected, and their expression levels were confirmed by quantitative RT-PCR (qRT-PCR). Thirteen of them exhibited expression patterns consistent with the digital gene expression (DGE) data. Conclusions: These sequencing datasets allowed us to elucidate the common and distinct mechanisms of plant responses to defoliation and wounding. Additionally, the distinct DEGs represent a valuable resource for novel gene discovery that may improve plant resistance to defoliation from various processes.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0089495
收录类别	SCI
语种	英语
WOS关键词	DOWN-REGULATION ; PERENNIAL RYEGRASS ; LOLIUM-PERENNE ; RNA-SEQ ; BIOSYNTHESIS ; EXPRESSION ; ARABIDOPSIS ; HERBIVORY ; LIGNIN ; GROWTH
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000331717900106
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Basic Research Program of China (973'') [2014CB138704] ; National High Technology Research and Development Program of China (863'') [2011AA100209] ; Ningxia Agricultural Comprehensive Development Office [NTKJ-2013-03 (1), NTKJ-2013-04] ; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-B-05]
作者邮箱	sychen@ibcas.ac.cn ; lixx258258@163.com ; liugs@ibcas.ac.cn
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：30[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27369
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Schoo1, Beijing, Peoples R China 3.Entry Exit Inspect & Quarantine Bur, Heze, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Shuangyan,Cai, Yueyue,Zhang, Lexin,et al. Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding[J]. PLOS ONE,2014,9(2).
APA	Chen, Shuangyan.,Cai, Yueyue.,Zhang, Lexin.,Yan, Xueqing.,Cheng, Liqin.,...&Liu, Gongshe.(2014).Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding.PLOS ONE,9(2).
MLA	Chen, Shuangyan,et al."Transcriptome Analysis Reveals Common and Distinct Mechanisms for Sheepgrass (Leymus chinensis) Responses to Defoliation Compared to Mechanical Wounding".PLOS ONE 9.2(2014).

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