Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery

doi:10.1186/1471-2229-14-110

IB-CAS > 中科院光生物学重点实验室

	Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery
	Liu, Guo-Tian ; Ma, Ling ; Duan, Wei ; Wang, Bai-Chen ; Li, Ji-Hu ; Xu, Hong-Guo ; Yan, Xue-Qing ; Yan, Bo-Fang ; Li, Shao-Hua; Wang, Li-Jun
	2014
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	14
摘要	Background: High temperature is a major environmental factor limiting grape yield and affecting berry quality. Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of the thermotolerance of Vitis, we combined a physiological analysis with iTRAQ-based proteomics of Vitis vinifera cv Cabernet Sauvignon, subjected to 43 degrees C for 6 h, and then followed by recovery at 25/18 degrees C. Results: High temperature increased the concentrations of TBARS and inhibited electronic transport in photosynthesis apparatus, indicating that grape leaves were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. One hundred and seventy-four proteins were differentially expressed under heat stress and/or during the recovery phase, in comparison to unstressed controls, respectively. Stress and recovery conditions shared 42 proteins, while 113 and 103 proteins were respectively identified under heat stress and recovery conditions alone. Based on MapMan ontology, functional categories for these dysregulated proteins included mainly photosynthesis (about 20%), proteins (13%), and stress (8%). The subcellular localization using TargetP showed most proteins were located in the chloroplasts (34%), secretory pathways (8%) and mitochondrion (3%). Conclusion: On the basis of these findings, we proposed that some proteins related to electron transport chain of photosynthesis, antioxidant enzymes, HSPs and other stress response proteins, and glycolysis may play key roles in enhancing grapevine adaptation to and recovery capacity from heat stress. These results provide a better understanding of the proteins involved in, and mechanisms of thermotolerance in grapevines.
关键词	Cabernet sauvignon Heat stress iTRAQ Photosynthesis Proteomics Recovery
学科领域	Plant Sciences
DOI	10.1186/1471-2229-14-110
收录类别	SCI
语种	英语
WOS关键词	PHOTOSYSTEM-II ; SHOCK PROTEINS ; SUBCELLULAR-LOCALIZATION ; MOLECULAR CHAPERONES ; CABERNET-SAUVIGNON ; HIGH-TEMPERATURE ; ABIOTIC STRESS ; WATER-DEFICIT ; OMICS DATA ; PLANTS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000335789900001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China [30771758, 31130047]
作者邮箱	ljwang@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：95[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27371
专题	中科院光生物学重点实验室
作者单位	1.[Liu, Guo-Tian 2.Ma, Ling 3.Duan, Wei 4.Li, Ji-Hu 5.Xu, Hong-Guo 6.Yan, Bo-Fang 7.Li, Shao-Hua 8.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 9.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China 10.Univ China Acad Sci, Beijing 100049, Peoples R China 11.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 12.Beijing Comp Ctr, Beijing 100094, Peoples R China 13.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Liu, Guo-Tian,Ma, Ling,Duan, Wei,et al. Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery[J]. BMC PLANT BIOLOGY,2014,14.
APA	Liu, Guo-Tian.,Ma, Ling.,Duan, Wei.,Wang, Bai-Chen.,Li, Ji-Hu.,...&Wang, Li-Jun.(2014).Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery.BMC PLANT BIOLOGY,14.
MLA	Liu, Guo-Tian,et al."Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery".BMC PLANT BIOLOGY 14(2014).

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