O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat

doi:10.1038/ncomms5572

	O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat
	Xiao, Jun 1; Xu, Shujuan 1; Li, Chunhua 1; Xu, Yunyuan ; Xing, Lijing ; Niu, Yuda ; Huan, Qing 1; Tang, Yimiao 2; Zhao, Changping 2; Wagner, Doris 3; Gao, Caixia 4; Chong, Kang 5
	2014
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
卷号	5
摘要	Vernalization, sensing of prolonged cold, is important for seasonal flowering in eudicots and monocots. While vernalization silences a repressor (FLC, MADS-box transcription factor) in eudicots, it induces an activator (TaVRN1, an AP1 clade MADS-box transcription factor) in monocots. The mechanism for TaVRN1 induction during vernalization is not well understood. Here we reveal a novel mechanism for controlling TaVRN1 mRNA accumulation in response to prolonged cold sensing in wheat. The carbohydrate-binding protein VER2, a jacalin lectin, promotes TaVRN1 upregulation by physically interacting with the RNA-binding protein TaGRP2. TaGRP2 binds to TaVRN1 pre-mRNA and inhibits TaVRN1 mRNA accumulation. The physical interaction between VER2 and TaGRP2 is controlled by TaGRP2 O-GlcNAc modification, which gradually increases during vernalization. The interaction between VER2 and O-GlcNAc-TaGRP2 reduces TaGRP2 protein accumulation in the nucleus and/or promotes TaGRP2 dissociation from TaVRN1, leading to TaVRN1 mRNA accumulation. Our data reveal a new mechanism for sensing prolonged cold in temperate cereals.
学科领域	Multidisciplinary Sciences
DOI	10.1038/ncomms5572
收录类别	SCI
语种	英语
WOS关键词	LOW-TEMPERATURE TOLERANCE ; N-ACETYLGLUCOSAMINE ; ARABIDOPSIS ; GENE ; PROTEIN ; TRANSFERASE ; RESPONSES ; LOCUS ; PHOSPHORYLATION ; EXPRESSION
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000341051700004
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
资助机构	major state basic research program of China (973) [2013CB126900, 2011CB915404] ; National Science Foundation of China [31121065]
作者邮箱	chongk@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：84[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27375
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Acad Agr & Forestry Sci, Hybrid Wheat Res Ctr, Beijing 100089, Peoples R China 4.Univ Penn, Dept Biol, Philadelphia, PA 19104 USA 5.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 6.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Jun,Xu, Shujuan,Li, Chunhua,et al. O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat[J]. NATURE COMMUNICATIONS,2014,5.
APA	Xiao, Jun.,Xu, Shujuan.,Li, Chunhua.,Xu, Yunyuan.,Xing, Lijing.,...&Chong, Kang.(2014).O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat.NATURE COMMUNICATIONS,5.
MLA	Xiao, Jun,et al."O-GlcNAc-mediated interaction between VER2 and TaGRP2 elicits TaVRN1 mRNA accumulation during vernalization in winter wheat".NATURE COMMUNICATIONS 5(2014).

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