Identification of a Retroelement from the Resurrection Plant Boea hygrometrica That Confers Osmotic and Alkaline Tolerance in Arabidopsis thaliana

doi:10.1371/journal.pone.0098098

	Identification of a Retroelement from the Resurrection Plant Boea hygrometrica That Confers Osmotic and Alkaline Tolerance in Arabidopsis thaliana
	Zhao, Yan ; Xu, Tao ; Shen, Chun-Ying ; Xu, Guang-Hui ; Chen, Shi-Xuan ; Song, Li-Zhen ; Li, Mei-Jing ; Wang, Li-Li ; Zhu, Yan ; Lv, Wei-Tao ; Gong, Zhi-Zhong ; Liu, Chun-Ming; Deng, Xin10
	2014
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	9 期号:5
摘要	Functional genomic elements, including transposable elements, small RNAs and non-coding RNAs, are involved in regulation of gene expression in response to plant stress. To identify genomic elements that regulate dehydration and alkaline tolerance in Boea hygrometrica, a resurrection plant that inhabits drought and alkaline Karst areas, a genomic DNA library from B. hygrometrica was constructed and subsequently transformed into Arabidopsis using binary bacterial artificial chromosome (BIBAC) vectors. Transgenic lines were screened under osmotic and alkaline conditions, leading to the identification of Clone L1-4 that conferred osmotic and alkaline tolerance. Sequence analyses revealed that L1-4 contained a 49-kb retroelement fragment from B. hygrometrica, of which only a truncated sequence was present in L1-4 transgenic Arabidopsis plants. Additional subcloning revealed that activity resided in a 2-kb sequence, designated Osmotic and Alkaline Resistance 1 (OAR1). In addition, transgenic Arabidopsis lines carrying an OAR1-homologue also showed similar stress tolerance phenotypes. Physiological and molecular analyses demonstrated that OAR1-transgenic plants exhibited improved photochemical efficiency and membrane integrity and biomarker gene expression under both osmotic and alkaline stresses. Short transcripts that originated from OAR1 were increased under stress conditions in both B. hygrometrica and Arabidopsis carrying OAR1. The relative copy number of OAR1 was stable in transgenic Arabidopsis under stress but increased in B. hygrometrica. Taken together, our results indicated a potential role of OAR1 element in plant tolerance to osmotic and alkaline stresses, and verified the feasibility of the BIBAC transformation technique to identify functional genomic elements from physiological model species.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0098098
收录类别	SCI
语种	英语
WOS关键词	GENOMIC DNA FRAGMENTS ; CRATEROSTIGMA-PLANTAGINEUM ; RAMONDA-SERBICA ; DEHYDRATION TOLERANCE ; DESICCATION TOLERANCE ; HABERLEA-RHODOPENSIS ; STRESS RESPONSES ; ABIOTIC STRESSES ; BIBAC LIBRARY ; SMALL RNAS
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000338323200045
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China [31270312, 30970431] ; National High Technology Research and Development Program of China (863 Program) [2007AA021403] ; National Basic Research Program of China (973 Program) [2012CB114302] ; Ministry of Agriculture of China [2009ZX08009-060B]
作者邮箱	deng@ibcas.ac.cn
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27377
专题	中科院植物分子生理学重点实验室
作者单位	1.[Zhao, Yan 2.Xu, Tao 3.Shen, Chun-Ying 4.Xu, Guang-Hui 5.Chen, Shi-Xuan 6.Song, Li-Zhen 7.Li, Mei-Jing 8.Wang, Li-Li 9.Zhu, Yan 10.Lv, Wei-Tao 11.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 12.[Song, Li-Zhen 13.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 14.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100094, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yan,Xu, Tao,Shen, Chun-Ying,et al. Identification of a Retroelement from the Resurrection Plant Boea hygrometrica That Confers Osmotic and Alkaline Tolerance in Arabidopsis thaliana[J]. PLOS ONE,2014,9(5).
APA	Zhao, Yan.,Xu, Tao.,Shen, Chun-Ying.,Xu, Guang-Hui.,Chen, Shi-Xuan.,...&Deng, Xin.(2014).Identification of a Retroelement from the Resurrection Plant Boea hygrometrica That Confers Osmotic and Alkaline Tolerance in Arabidopsis thaliana.PLOS ONE,9(5).
MLA	Zhao, Yan,et al."Identification of a Retroelement from the Resurrection Plant Boea hygrometrica That Confers Osmotic and Alkaline Tolerance in Arabidopsis thaliana".PLOS ONE 9.5(2014).

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