The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus

doi:10.1186/1471-2180-14-95

	The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus
	Zhang, Jin-Dan ; Han, Lida ; Yan, Shijuan ; Liu, Chun-Ming
	2014
发表期刊	BMC MICROBIOLOGY
ISSN	1471-2180
卷号	14
摘要	Background: Aflatoxins (AFs) are potent carcinogenic compounds produced by several Aspergillus species, which pose serious threats to human health. As sugar is a preferred carbohydrate source for AF production, we examined the possibility of using sugar analogs to inhibit AF biosynthesis. Results: We showed that although D-glucal cannot be utilized by A. flavus as the sole carbohydrate source, it inhibited AF biosynthesis and promoted kojic acid production without affecting mycelial growth when applied to a glucose-containing medium. The inhibition occurred before the production of the first stable intermediate, norsolorinic acid, suggesting a complete inhibition of the AF biosynthetic pathway. Further studies showed that exogenous D-glucal in culture led to reduced accumulation of tricarboxylic acid (TCA) cycle intermediates and reduced glucose consumption, indicating that glycolysis is inhibited. Expression analyses revealed that D-glucal suppressed the expression of AF biosynthetic genes but promoted the expression of kojic acid biosynthetic genes. Conclusions: D-glucal as a non-metabolizable glucose analog inhibits the AF biosynthesis pathway by suppressing the expression of AF biosynthetic genes. The inhibition may occur either directly through interfering with glycolysis, or indirectly through reduced oxidative stresses from kojic acid biosynthesis.
关键词	D-glucal D-galactal Aflatoxin biosynthesis Aspergillus flavus Metabolomics
学科领域	Microbiology
DOI	10.1186/1471-2180-14-95
收录类别	SCI
语种	英语
WOS关键词	CARBON-SOURCES ; RESTING CELLS ; PARASITICUS ; GENE ; SPORULATION ; METABOLISM ; GENOMICS ; PEPTONE ; PATHWAY ; OXIDASE
WOS研究方向	Microbiology
WOS记录号	WOS:000335416300002
出版者	BIOMED CENTRAL LTD
文献子类	Article
出版地	LONDON
资助机构	Key Innovation Project [KSCX2-YW-N-033] ; Chinese Academy of Sciences
作者邮箱	cmliu@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27378
专题	中科院植物分子生理学重点实验室
作者单位	1.[Zhang, Jin-Dan 2.Han, Lida 3.Yan, Shijuan 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jin-Dan,Han, Lida,Yan, Shijuan,et al. The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus[J]. BMC MICROBIOLOGY,2014,14.
APA	Zhang, Jin-Dan,Han, Lida,Yan, Shijuan,&Liu, Chun-Ming.(2014).The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus.BMC MICROBIOLOGY,14.
MLA	Zhang, Jin-Dan,et al."The non-metabolizable glucose analog D-glucal inhibits aflatoxin biosynthesis and promotes kojic acid production in Aspergillus flavus".BMC MICROBIOLOGY 14(2014).

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