The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis

doi:10.1186/1471-2229-13-225

	The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis
	Xu, Ting-Ting ; Song, Xiu-Fen ; Ren, Shi-Chao ; Liu, Chun-Ming
	2013
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	13
摘要	Background: Although it is known that CLAVATA3 (CLV3) acts as 12-and/ or 13-amino acid (AA) secreted peptides to regulate the number of stem cells in shoot apical meristems (SAMs), how functional CLV3 peptides are generated and if any particular sequences are required for the processing remain largely unknown. Results: We developed a mass spectrometry (MS)-based in vitro assay to monitor the cleavage of heterologously produced CLV3 fusion protein. Through co-cultivation of the fusion protein with Arabidopsis seedlings, we identified two cleavage sites: the previously reported one before Arg70 and a new one before Met39. Using synthetic peptides together with MALDI-Tof-MS analyses, we demonstrated that the non-conserved 5-AA motifs flanking N-termini of the CLV3 and its orthologous CLE1 peptides were critical for their cleavages and optimal activities in vitro. We also found that substitutions of Leu69 by Ala in fusion protein and in synthetic peptide of CLV3 compromised their cleavages, leading to significantly reduced activities in regulating the sizes of shoot and root meristems. Conclusions: These results suggest that 5-AA residues flanking the N-terminus of CLV3 peptide are required for proper cleavages and optimal function in stem cell regulation.
关键词	CLV3 Peptide cleavage Flanking sequence AA substitution Stem cell regulation
学科领域	Plant Sciences
DOI	10.1186/1471-2229-13-225
收录类别	SCI
语种	英语
WOS关键词	SECRETORY PEPTIDE ; RECEPTOR KINASE ; SMALL PROTEIN ; GENE ENCODES ; GROWTH ; POLYPEPTIDE ; CLAVATA3 ; MERISTEM ; SIGNAL ; FATE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000329090000001
出版者	BMC
文献子类	Article
出版地	LONDON
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Science and Technology of China(Ministry of Science and Technology, China)
作者邮箱	刘, 春明/GLU-4320-2022 ; Liu, Chun-Ming/G-1006-2010
作品OA属性	Green Published, gold, Green Accepted
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27781
专题	中科院植物分子生理学重点实验室
作者单位	1.[Xu, Ting-Ting 2.Song, Xiu-Fen 3.Ren, Shi-Chao 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,et al. The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis[J]. BMC PLANT BIOLOGY,2013,13.
APA	Xu, Ting-Ting,Song, Xiu-Fen,Ren, Shi-Chao,&Liu, Chun-Ming.(2013).The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis.BMC PLANT BIOLOGY,13.
MLA	Xu, Ting-Ting,et al."The sequence flanking the N-terminus of the CLV3 peptide is critical for its cleavage and activity in stem cell regulation in Arabidopsis".BMC PLANT BIOLOGY 13(2013).

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