Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis

doi:10.1371/journal.pone.0075773

	Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis
	Zhang, Dajian 1; Wang, Xiaomin ; Wang, Min ; Li, Junhua ; Guo, Xiaoyu ; Chong, Kang ; Xu, Yunyuan
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:9
摘要	WUSCHEL (WUS) is essential for preventing stem cell differentiation in Arabidopsis. Here we report that in addition to its functions in meristematic stem cell maintenance, WUS is involved in the regulation of cell division. The WUS gain-of-function mutant, stem ectopic flowers (sef), displayed elongated hypocotyls, whereas the loss-of-function wus-1 mutant had shortened hypocotyls. The long hypocotyl in sef was due to the presence of more cells, rather than increased cell elongation. Microscopic observation, flow cytometry assays, quantitative RT-PCR (qRT-PCR), and histochemical staining of CycB1;1::GUS supported the hypothesis that ectopic cell division occurred in the sef hypocotyls after germination. Both immunoblot and qRT-PCR results showed that WUS was ectopically expressed in sef hypocotyls. Luciferase activity, chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assay (EMSA) showed that GLUTAMINE-RICH PROTEIN 23 (GRP23) expression can be activated by WUS and that GRP23 is a direct target gene of WUS. The phenotypes of 35S::GRP23 plants and GRP23 knockdown lines supported the notion that GRP23 mediates the effects of WUS on hypocotyl length. Together, our data suggest that ectopic expression of WUS in hypocotyl controls cell division through its target gene GRP23.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0075773
收录类别	SCI
语种	英语
WOS关键词	WUSCHEL GENE ; SHOOT ; CYTOKININ ; PROTEIN ; GROWTH ; AUXIN ; CYCLE ; FATE ; GIBBERELLINS ; TOLERANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000325220000043
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Science Foundation of China for Innovative Research Groups(National Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars)
作品OA属性	gold, Green Accepted, Green Published, Green Submitted
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27785
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dajian,Wang, Xiaomin,Wang, Min,et al. Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis[J]. PLOS ONE,2013,8(9).
APA	Zhang, Dajian.,Wang, Xiaomin.,Wang, Min.,Li, Junhua.,Guo, Xiaoyu.,...&Xu, Yunyuan.(2013).Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis.PLOS ONE,8(9).
MLA	Zhang, Dajian,et al."Ectopic Expression of WUS in Hypocotyl Promotes Cell Division via GRP23 in Arabidopsis".PLOS ONE 8.9(2013).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Zhang-2013-Ectopic E（2370KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文