RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis

doi:10.1371/journal.pone.0072914

	RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis
	Gao, Bin ; Fan, Lusheng 1; Li, Xingxing ; Yang, Huifang ; Liu, Fengluan ; Wang, Ling ; Xi, Lin ; Ma, Nan ; Zhao, Liangjun
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:8
摘要	In vitro, a new protocol of plant regeneration in rose was achieved via protocorm-like bodies (PLBs) induced from the rootlike organs named rhizoids that developed from leaf explants. The development of rhizoids is a critical stage for efficient regeneration, which is triggered by exogenous auxin. However, the role of cytokinin in the control of organogenesis in rose is as yet uncharacterized. The aim of this study was to elucidate the molecular mechanism of cytokinin-modulated rhizoid formation in Rosa canina. Here, we found that cytokinin is a key regulator in the formation of rhizoids. Treatment with cytokinin reduced callus activity and significantly inhibited rhizoid formation in Rosa canina. We further isolated the full-length cDNA of a type-A response regulator gene of cytokinin signaling, RcRR1, from which the deduced amino acid sequence contained the conserved DDK motif. Gene expression analysis revealed that RcRR1 was differentially expressed during rhizoid formation and its expression level was rapidly up-regulated by cytokinin. In addition, the functionality of RcRR1 was tested in Arabidopsis. RcRR1 was found to be localized to the nucleus in GFP-RcRR1 transgenic plants and overexpression of RcRR1 resulted in increased primary root length and lateral root density. More importantly, RcRR1 overexpression transgenic plants also showed reduced sensitivity to cytokinin during root growth; auxin distribution and the expression of auxin efflux carriers PIN genes were altered in RcRR1 overexpression plants. Taken together, these results demonstrate that RcRR1 is a functional type-A response regulator which is involved in cytokinin-regulated rhizoid formation in Rosa canina.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0072914
收录类别	SCI
语种	英语
WOS关键词	IN-VITRO PROPAGATION ; SIGNAL-TRANSDUCTION ; PLANT-REGENERATION ; LEAF EXPLANTS ; ARABIDOPSIS ; BIOSYNTHESIS ; EXPRESSION ; PROTEINS ; GROWTH ; SIZE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323734600042
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作品OA属性	gold, Green Submitted, Green Published, Green Accepted
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27788
专题	中科院植物分子生理学重点实验室
作者单位	1.China Agr Univ, Dept Ornamental Hort & Landscape Architecture, Beijing 100094, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Gao, Bin,Fan, Lusheng,Li, Xingxing,et al. RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis[J]. PLOS ONE,2013,8(8).
APA	Gao, Bin.,Fan, Lusheng.,Li, Xingxing.,Yang, Huifang.,Liu, Fengluan.,...&Zhao, Liangjun.(2013).RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis.PLOS ONE,8(8).
MLA	Gao, Bin,et al."RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis".PLOS ONE 8.8(2013).

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