Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth

doi:10.1371/journal.pone.0055411

	Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth
	Wang, Li 1; Lv, Xueqin 2; Li, Hong 2; Zhang, Min 1; Wang, Hong ; Jin, Biao 1; Chen, Tong
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:2
摘要	Calmodulin (CaM) is one of the most well-studied Ca2+ transducers in eukaryotic cells. It is known to regulate the activity of numerous proteins with diverse cellular functions; however, the functions of apoplastic CaM in plant cells are still poorly understood. By combining pharmacological analysis and microscopic techniques, we investigated the involvement of apoplastic CaM in pollen tube growth of Cedrus deodara (Roxb.) Loud. It was found that the tip-focused calcium gradient was rapidly disturbed as one of the early events after application of pharmacological agents, while the cytoplasmic organization was not significantly affected. The deposition and distribution of acidic pectins and esterified pectins were also dramatically changed, further perturbing the normal modeling of the cell wall. Several protein candidates from different functional categories may be involved in the responses to inhibition of apoplastic CaM. These results revealed that apoplastic CaM functions to maintain the tip-focused calcium gradient and to modulate the distribution/transformation of pectins during pollen tube growth.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0055411
收录类别	SCI
语种	英语
WOS关键词	EXTRACELLULAR CALMODULIN ; SIGNAL-TRANSDUCTION ; HIGHER-PLANTS ; CARROT CELLS ; GERMINATION ; PROTEIN ; GRADIENT ; PECTIN ; CA2+ ; POLYPEPTIDE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000315153400093
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Natural Science Foundation of Jiangsu Province(Natural Science Foundation of Jiangsu Province)
作者邮箱	Chen, Tong/C-5684-2014
作品OA属性	Green Published, Green Accepted, Green Submitted, gold
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27793
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 2.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou, Jiangsu, Peoples R China 3.Yangzhou Univ, Coll Biol Sci & Technol, Yangzhou, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,Lv, Xueqin,Li, Hong,et al. Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth[J]. PLOS ONE,2013,8(2).
APA	Wang, Li.,Lv, Xueqin.,Li, Hong.,Zhang, Min.,Wang, Hong.,...&Chen, Tong.(2013).Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth.PLOS ONE,8(2).
MLA	Wang, Li,et al."Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth".PLOS ONE 8.2(2013).

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