Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes

doi:10.1371/journal.pone.0058681

IB-CAS > 中科院光生物学重点实验室

	Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes
	Liu, Chang-Cai ; Zhu, Hang-Yong ; Dong, Xiu-Mei ; Ning, De-Li ; Wang, Hong-Xia ; Li, Wei-Hua ; Yang, Chuan-Ping ; Wang, Bai-Chen
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:3
摘要	Background: The N-terminal protein processing mechanism (NPM) including N-terminal Met excision (NME) and N-terminal acetylation (N-alpha-acetylation) represents a common protein co-translational process of some eukaryotes. However, this NPM occurred in woody plants yet remains unknown. Methodology/Principal Findings: To reveal the NPM in poplar, we investigated the N-alpha-acetylation status of poplar proteins during dormancy by combining tandem mass spectrometry with TiO2 enrichment of acetylated peptides. We identified 58 N-terminally acetylated (N-alpha-acetylated) proteins. Most proteins (47, >81%) are subjected to N-alpha-acetylation following the N-terminal removal of Met, indicating that N-alpha-acetylation and NME represent a common NPM of poplar proteins. Furthermore, we confirm that poplar shares the analogous NME and N-alpha-acetylation (NPM) to other eukaryotes according to analysis of N-terminal features of these acetylated proteins combined with genome-wide identification of the involving methionine aminopeptidases (MAPs) and N-terminal acetyltransferase (Nat) enzymes in poplar. The N-alpha-acetylated reactions and the involving enzymes of these poplar proteins are also identified based on those of yeast and human, as well as the subcellular location information of these poplar proteins. Conclusions/Significance: This study represents the first extensive investigation of N-alpha-acetylation events in woody plants, the results of which will provide useful resources for future unraveling the regulatory mechanisms of N-alpha-acetylation of proteins in poplar.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0058681
收录类别	SCI
语种	英语
WOS关键词	HIGHLY SELECTIVE ENRICHMENT ; ALPHA-ACETYLTRANSFERASE ; PHOSPHORYLATED PEPTIDES ; METHIONINE EXCISION ; YEAST ; DYNAMICS ; GENOME ; IMPACT ; STROMA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000316251100079
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Basic Research Priorities Program(National Basic Research Program of China) ; State Key Program of the National Natural Science of China(National Natural Science Foundation of China (NSFC)) ; Northeast Forestry University
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27795
专题	中科院光生物学重点实验室
作者单位	1.[Liu, Chang-Cai 2.Zhu, Hang-Yong 3.Dong, Xiu-Mei 4.Ning, De-Li 5.Yang, Chuan-Ping 6.Northeast Forestry Univ, State Key Lab Forest Genet & Tree Breeding, Harbin, Heilongjiang, Peoples R China 7.Hubei Nanxing Gen Chem Factory, Lab Chem Def & Microscale Anal, Zhijiang, Hubei, Peoples R China 8.Bur Garden & Pk, Qitaihe, Heilongjiang, Peoples R China 9.[Wang, Hong-Xia 10.Natl Ctr Biomed Anal, Inst Basic Med Sci, Beijing, Peoples R China 11.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Chang-Cai,Zhu, Hang-Yong,Dong, Xiu-Mei,et al. Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes[J]. PLOS ONE,2013,8(3).
APA	Liu, Chang-Cai.,Zhu, Hang-Yong.,Dong, Xiu-Mei.,Ning, De-Li.,Wang, Hong-Xia.,...&Wang, Bai-Chen.(2013).Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes.PLOS ONE,8(3).
MLA	Liu, Chang-Cai,et al."Identification and Analysis of the Acetylated Status of Poplar Proteins Reveals Analogous N-Terminal Protein Processing Mechanisms with Other Eukaryotes".PLOS ONE 8.3(2013).

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