Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress

doi:10.1371/journal.pone.0058740

	Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress
	Xin, Haiping 1; Zhu, Wei 2; Wang, Lina 2; Xiang, Yue 2; Fang, Linchuan 2; Li, Jitao 2; Sun, Xiaoming 2; Wang, Nian 1; Londo, Jason P.; Li, Shaohua1
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:3
摘要	Grape is one of the most important fruit crops worldwide. The suitable geographical locations and productivity of grapes are largely limited by temperature. Vitis amurensis is a wild grapevine species with remarkable cold-tolerance, exceeding that of Vitis vinifera, the dominant cultivated species of grapevine. However, the molecular mechanisms that contribute to the enhanced freezing tolerance of V. amurensis remain unknown. Here we used deep sequencing data from restriction endonuclease-generated cDNA fragments to evaluate the whole genome wide modification of transcriptome of V. amurensis under cold treatment. Vitis vinifera cv. Muscat of Hamburg was used as control to help investigate the distinctive features of V. amruensis in responding to cold stress. Approximately 9 million tags were sequenced from non-cold treatment (NCT) and cold treatment (CT) cDNA libraries in each species of grapevine sampled from shoot apices. Alignment of tags into V. vinifera cv. Pinot noir (PN40024) annotated genome identified over 15,000 transcripts in each library in V. amruensis and more than 16,000 in Muscat of Hamburg. Comparative analysis between NCT and CT libraries indicate that V. amurensis has fewer differential expressed genes (DEGs, 1314 transcripts) than Muscat of Hamburg (2307 transcripts) when exposed to cold stress. Common DEGs (408 transcripts) suggest that some genes provide fundamental roles during cold stress in grapes. The most robust DEGs (more than 20-fold change) also demonstrated significant differences between two kinds of grapevine, indicating that cold stress may trigger species specific pathways in V. amurensis. Functional categories of DEGs indicated that the proportion of up-regulated transcripts related to metabolism, transport, signal transduction and transcription were more abundant in V. amurensis. Several highly expressed transcripts that were found uniquely accumulated in V. amurensis are discussed in detail. This subset of unique candidate transcripts may contribute to the excellent cold-hardiness of V. amurensis.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0058740
收录类别	SCI
语种	英语
WOS关键词	LOW-TEMPERATURE ; FREEZING TOLERANCE ; ABSCISIC-ACID ; GENE-EXPRESSION ; PROTEIN-KINASE ; ABIOTIC STRESS ; ARABIDOPSIS ; DROUGHT ; GRAPEVINE ; ACCLIMATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000316849200108
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China (NSFC)(National Natural Science Foundation of China (NSFC))
作者邮箱	Zhu, Wei/HGA-4471-2022 ; Sun, Xiaoming/AHA-6034-2022 ; fang, lin chuan/K-4923-2013
作品OA属性	Green Published, Green Submitted, gold, Green Accepted
引用统计	被引频次：84[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27802
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Bot, Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Londo, Jason P.] ARS, USDA, Grape Genet Res Unit, Geneva, NY USA
推荐引用方式 GB/T 7714	Xin, Haiping,Zhu, Wei,Wang, Lina,et al. Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress[J]. PLOS ONE,2013,8(3).
APA	Xin, Haiping.,Zhu, Wei.,Wang, Lina.,Xiang, Yue.,Fang, Linchuan.,...&Li, Shaohua.(2013).Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress.PLOS ONE,8(3).
MLA	Xin, Haiping,et al."Genome Wide Transcriptional Profile Analysis of Vitis amurensis and Vitis vinifera in Response to Cold Stress".PLOS ONE 8.3(2013).

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