Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies

doi:10.1371/journal.pone.0079996

	Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies
	Zhou, Hua 1; Cheng, Fang-Yun ; Wang, Rong ; Zhong, Yuan ; He, Chaoying2
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:11
摘要	Tree peonies are important ornamental plants worldwide, but growing them can be frustrating due to their short and concentrated flowering period. Certain cultivars exhibit a reblooming trait that provides a valuable alternative for extending the flowering period. However, the genetic control of reblooming in tree peonies is not well understood. In this study, we compared the molecular properties and morphology of reblooming and non-reblooming tree peonies during the floral initiation and developmental processes. Using transcriptome sequencing technology, we generated 59,275 and 63,962 unigenes with a mean size of 698 bp and 699 bp from the two types of tree peonies, respectively, and identified eight differentially expressed genes that are involved in the floral pathways of Arabidopsis thaliana. These differentially regulated genes were verified through a detailed analysis of their expression pattern during the floral process by real time RT-PCR. From this combined analysis, we identified four genes, PsFT, PsVIN3, PsCO and PsGA20OX, which likely play important roles in the regulation of the reblooming process in tree peonies. These data constitute a valuable resource for the discovery of genes involved in flowering time and insights into the molecular mechanism of flowering to further accelerate the breeding of tree peonies and other perennial woody plants.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0079996
收录类别	SCI
语种	英语
WOS关键词	FLOWERING-LOCUS-T ; RNA-SEQ ; MOLECULAR-MECHANISM ; GIBBERELLIN ; TIME ; ARABIDOPSIS ; TOOL ; VERNALIZATION ; ANNOTATION ; TRANSITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000327143800107
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National High Technology Research and Development Program of China (863 Program)(National High Technology Research and Development Program of China)
作品OA属性	Green Accepted, Green Published, gold, Green Submitted
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27849
专题	系统与进化植物学国家重点实验室
作者单位	1.Beijing Forestry Univ, Landscape Architecture Coll, Natl Flower Engn Res Ctr, Beijing, Peoples R China 2.Jiangxi Acad Sci, Inst Biol & Resources, Nanchang, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Hua,Cheng, Fang-Yun,Wang, Rong,et al. Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies[J]. PLOS ONE,2013,8(11).
APA	Zhou, Hua,Cheng, Fang-Yun,Wang, Rong,Zhong, Yuan,&He, Chaoying.(2013).Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies.PLOS ONE,8(11).
MLA	Zhou, Hua,et al."Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies".PLOS ONE 8.11(2013).

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