Genetic Features of the Spontaneous Self-Compatible Mutant, 'Jin Zhui' (Pyrus bretschneideri Rehd.)

doi:10.1371/journal.pone.0076509

	Genetic Features of the Spontaneous Self-Compatible Mutant, 'Jin Zhui' (Pyrus bretschneideri Rehd.)
	Wu, Junkai ; Li, Maofu 1; Li, Tianzhong
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:10
摘要	'Jin Zhui' is a spontaneous self-compatible mutant of 'Ya Li' (Pyrus bretschneideri Rehd. S21S34), the latter displaying a typical S-RNase-based gametophytic self-incompatibility (GSI). The pollen-part mutation (PPM) of 'Jin Zhui' might be due to a natural mutation in the pollen-S gene (S-34 haplotype). However, the molecular mechanisms behind these phenotypic changes are still unclear. In this study, we identified five SLF (S-Locus F-box) genes in 'Ya Li', while no nucleotide differences were found in the SLF genes of 'Jin Zhui'. Further genetic analysis by S-RNase PCR-typing of selfed progeny of 'Jin Zhui' and 'Ya Li' x 'Jin Zhui' progeny showed three progeny classes (S21S21, S21S34 and S34S34) as opposed to the two classes reported previously (S21S34 and S34S34), indicating that the pollen gametes of 'Jin Zhui', bearing either the S-21- or S-34-haplotype, were able to overcome self-incompatibility (SI) barriers. Moreover, no evidence of pollen-S duplication was found. These findings support the hypothesis that loss of function of S-locus unlinked PPM expressed in pollen leads to SI breakdown in 'Jin Zhui', rather than natural mutation in the pollen-S gene (S-34 haplotype). Furthermore, abnormal meiosis was observed in a number of pollen mother cells (PMCs) in 'Jin Zhui', but not in 'Ya Li'. These and other interesting findings are discussed.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0076509
收录类别	SCI
语种	英语
WOS关键词	F-BOX GENE ; INCOMPATIBILITY S-LOCUS ; SKP1-LIKE PROTEIN ; NICOTIANA-ALATA ; PRUNUS-AVIUM ; POLLEN ; RNASE ; IDENTIFICATION ; MULTIPLE ; RECOGNITION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000325501300060
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	Beijing Natural Science Foundation(Beijing Natural Science Foundation) ; National Science Foundation for Young Scholars of China(National Natural Science Foundation of China (NSFC))
作品OA属性	gold, Green Published, Green Submitted
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27854
专题	系统与进化植物学国家重点实验室
作者单位	1.China Agr Univ, Lab Fruit Tree Cell & Mol Breeding, Beijing 100094, Peoples R China 2.Chinese Acad Sci, State Key Lab Systemat & Evolutionary Bot, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Junkai,Li, Maofu,Li, Tianzhong. Genetic Features of the Spontaneous Self-Compatible Mutant, 'Jin Zhui' (Pyrus bretschneideri Rehd.)[J]. PLOS ONE,2013,8(10).
APA	Wu, Junkai,Li, Maofu,&Li, Tianzhong.(2013).Genetic Features of the Spontaneous Self-Compatible Mutant, 'Jin Zhui' (Pyrus bretschneideri Rehd.).PLOS ONE,8(10).
MLA	Wu, Junkai,et al."Genetic Features of the Spontaneous Self-Compatible Mutant, 'Jin Zhui' (Pyrus bretschneideri Rehd.)".PLOS ONE 8.10(2013).

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