Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes

doi:10.1371/journal.pone.0076500

	Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes
	Zhang, Ximei 1; Liu, Wei 2; Schloter, Michael 3; Zhang, Guangming 1; Chen, Quansheng 1; Huang, Jianhui1 ; Li, Linghao1 ; Elser, James J.; Han, Xingguo1
	2013
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	8 期号:10
摘要	Multiple co-occurring environmental changes are affecting soil nitrogen cycling processes, which are mainly mediated by microbes. While it is likely that various nitrogen-cycling functional groups will respond differently to such environmental changes, very little is known about their relative responsiveness. Here we conducted four long-term experiments in a steppe ecosystem by removing plant functional groups, mowing, adding nitrogen, adding phosphorus, watering, warming, and manipulating some of their combinations. We quantified the abundance of seven nitrogen-cycling genes, including those for fixation (nifH), mineralization (chiA), nitrification (amoA of ammonia-oxidizing bacteria (AOB) or archaea (AOA)), and denitrification (nirS, nirK and nosZ). First, for each gene, we compared its sensitivities to different environmental changes and found that the abundances of various genes were sensitive to distinct and different factors. Overall, the abundances of nearly all genes were sensitive to nitrogen enrichment. In addition, the abundances of the chiA and nosZ genes were sensitive to plant functional group removal, the AOB-amoA gene abundance to phosphorus enrichment when nitrogen was added simultaneously, and the nirS and nirK gene abundances responded to watering. Second, for each single-or multifactorial environmental change, we compared the sensitivities of the abundances of different genes and found that different environmental changes primarily affected different gene abundances. Overall, AOB-amoA gene abundance was most responsive, followed by the two denitrifying genes nosZ and nirS, while the other genes were less sensitive. These results provide, for the first time, systematic insights into how the abundance of each type of nitrogen-cycling gene and the equilibrium state of all these nitrogen-cycling gene abundances would shift under each single-or multi-factorial global change.
学科领域	Multidisciplinary Sciences
DOI	10.1371/journal.pone.0076500
收录类别	SCI
语种	英语
WOS关键词	AMMONIA-OXIDIZING BACTERIA ; DENITRIFYING BACTERIA ; TERRESTRIAL ECOSYSTEMS ; COMMUNITIES ; BIODIVERSITY ; DEPOSITION ; DIVERSITY ; STEPPE ; DENITRIFICATION ; TRANSFORMATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000325489100125
出版者	PUBLIC LIBRARY SCIENCE
文献子类	Article
出版地	SAN FRANCISCO
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; State Key Basic Research Development Program of China (973 Program)(National Basic Research Program of China) ; Postdoctoral Science Foundation of China(China Postdoctoral Science Foundation) ; US National Science Foundation(National Science Foundation (NSF))
作者邮箱	Elser, James J/AAL-6199-2020 ; Schloter, 1Michael/H-9151-2013 ; Elser, James/A-7082-2008 ; Han, Xingguo/K-7552-2016 ; Chen, Quansheng/AGH-8083-2022
作品OA属性	gold, Green Published, Green Accepted, Green Submitted
引用统计	被引频次：49[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27855
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, State Key Lab Forest & Soil Ecol, Inst Appl Ecol, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, State Key Lab Vegetat & Environm Change, Inst Bot, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Helmholtz Ctr Environm Hlth, Oberschleissheim, Germany 5.Elser, James J.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85069 USA
推荐引用方式 GB/T 7714	Zhang, Ximei,Liu, Wei,Schloter, Michael,et al. Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes[J]. PLOS ONE,2013,8(10).
APA	Zhang, Ximei.,Liu, Wei.,Schloter, Michael.,Zhang, Guangming.,Chen, Quansheng.,...&Han, Xingguo.(2013).Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes.PLOS ONE,8(10).
MLA	Zhang, Ximei,et al."Response of the Abundance of Key Soil Microbial Nitrogen-Cycling Genes to Multi-Factorial Global Changes".PLOS ONE 8.10(2013).

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