Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach

doi:10.1021/es4012096

	Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach
	Chen, Yanshan 1; Xu, Wenzhong ; Shen, Hongling 1; Yan, Huili 1; Xu, Wenxiu ; He, Zhenyan; Ma, Mi
	2013
发表期刊	ENVIRONMENTAL SCIENCE & TECHNOLOGY
ISSN	0013-936X
卷号	47 期号:16 页码:9355-9362
摘要	Arsenic (As) pollution is a global problem, and the plant-based cleanup of contaminated soils, called phytoremediation, is therefore of great interest. Recently, transgenic approaches have been designed to develop As phytoremediation technologies. Here, we used a one-gene transgenic approach for As tolerance and accumulation in Arabidopsis thaliana. PvACR3, a key arsenite [As(III)] antiporter in the As hyperaccumulator fern Pteris vittata, was expressed in Arabidopsis, driven by the CaMV 35S promoter. In response to As treatment, PvACR3 transgenic plants showed greatly enhanced tolerance. PvACR3 transgenic seeds could even germinate and grow in the presence of 80 mu M As(III) or 1200 mu M arsenate [As(V)] treatments that were lethal to wildtype seeds. PvACR3 localizes to the plasma membrane in Arabidopsis and increases arsenite efflux into external medium in short-term experiments. Arsenic determination showed that PvACR3 substantially reduced As concentrations in roots and simultaneously increased shoot As under 150 mu M As(V). When cultivated in As(V)-containing soil (10 ppm As), transgenic plants accumulated approximately 7.5-fold more As in above-ground tissues than wild-type plants. This study provides important insights into the behavior of PvACR3 and the physiology of As metabolism in plants. Our work also provides a simple and practical PvACR3 transgenic approach for engineering As-tolerant and -hyperaccumulating plants for phytoremediation.
学科领域	Engineering, Environmental ; Environmental Sciences
DOI	10.1021/es4012096
收录类别	SCI
语种	英语
WOS关键词	PTERIS-VITTATA ; ACCUMULATION ; ARABIDOPSIS ; EFFLUX ; RICE ; FERN ; REDUCTION ; CADMIUM ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000323471700045
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
EISSN	1520-5851
资助机构	National Science Foundation Council of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Chen, Yanshan/F-1830-2017
引用统计	被引频次：91[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27888
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yanshan,Xu, Wenzhong,Shen, Hongling,et al. Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2013,47(16):9355-9362.
APA	Chen, Yanshan.,Xu, Wenzhong.,Shen, Hongling.,Yan, Huili.,Xu, Wenxiu.,...&Ma, Mi.(2013).Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach.ENVIRONMENTAL SCIENCE & TECHNOLOGY,47(16),9355-9362.
MLA	Chen, Yanshan,et al."Engineering Arsenic Tolerance and Hyperaccumulation in Plants for Phytoremediation by a PvACR3 Transgenic Approach".ENVIRONMENTAL SCIENCE & TECHNOLOGY 47.16(2013):9355-9362.

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