Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice

doi:10.1016/j.jplph.2012.08.019

	Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice
	Chen, Yuan 1; Li, Fei 1; Ma, Yan 1; Chong, Kang ; Xu, Yunyuan
	2013
发表期刊	JOURNAL OF PLANT PHYSIOLOGY
ISSN	0176-1617
卷号	170 期号:1 页码:93-100
摘要	The basic helix-loop-helix family of proteins, which function as transcription factors, have been intensively studied in plants and animals. However, the molecular mechanism of these factors contributing to stress tolerance is unknown. Here, We report on the overexpression of OrbHLH001 from Dongxiang wild rice (Oryza rufipogon) conferring salt tolerance in transgenic rice plants. The expression of OrbHLH001 was tissue specific, mainly in phloem tissues throughout the plant. Ion assay with the scanning ion-selective electrode technique showed that NaCl stress has a greater influence on Na+ efflux and K+ influx in OrbHLH001-overexpressed plants than the wild type. OrbHLH001 protein can induce the expression of OsAKT1 to regulate the Na+/K+ ratio in OrbHLH001-overexpressed plants by specifically binding to an E-box motif in the promoter region of OsAKT1. The Mechanism may have potential use in rice molecular breeding. (c) 2012 Elsevier GmbH. All rights reserved.
关键词	OrbHLH001 Transcription factors Ion flux E-box Salt tolerance
学科领域	Plant Sciences
DOI	10.1016/j.jplph.2012.08.019
收录类别	SCI
语种	英语
WOS关键词	K+ CHANNEL ; TRANSGENIC RICE ; TOLERANCE ; COLD ; GENE ; DROUGHT ; DOMAIN ; DEHYDRATION ; REGULATOR ; RESPONSES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000314085200013
出版者	ELSEVIER GMBH
文献子类	Article
出版地	MUNICH
EISSN	1618-1328
资助机构	Research Program from the Chinese Ministry of Agriculture ; Innovation Grants of the Chinese Academy of Sciences
作者邮箱	MA, YAN/HHN-2912-2022
引用统计	被引频次：43[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27890
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yuan,Li, Fei,Ma, Yan,et al. Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(1):93-100.
APA	Chen, Yuan,Li, Fei,Ma, Yan,Chong, Kang,&Xu, Yunyuan.(2013).Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice.JOURNAL OF PLANT PHYSIOLOGY,170(1),93-100.
MLA	Chen, Yuan,et al."Overexpression of OrbHLH001, a putative helix-loop-helix transcription factor, causes increased expression of AKT1 and maintains ionic balance under salt stress in rice".JOURNAL OF PLANT PHYSIOLOGY 170.1(2013):93-100.

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