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| Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region | |
| Gong, Deshun1; Yang, Fan1; Li, Fudong1; Qian, Dandan2; Wu, Minhao1; Shao, Zhenhua1; Wu, Mian1; Wu, Jihui1; Shi, Yunyu1 | |
| 2013 | |
| 发表期刊 | BIOCHEMICAL JOURNAL
 |
| ISSN | 0264-6021 |
| 卷号 | 450页码:85-94 |
| 摘要 | Human RBM25 (RNA-binding motif protein 25) is a novel splicing factor that contains a PWI domain, a newly identified RNA/DNA-binding domain, and regulates Bcl-x pre-mRNA alternative splicing. The flanking basic region has been suggested to serve as a co-operative partner of the PWI domain in the binding of nucleic acids, but the structure of this basic region is unknown. In the present paper, we report the crystal structure of the RBM25 PWI domain and its flanking basic region. The PWI domain is revealed to comprise a conserved four-helix bundle, and the flanking basic region forms two a-helices and associates with helix H4 of the PWI domain. These interactions promote directly the formation of an enlarged nucleic-acid-binding platform. Structure-guided mutagenesis reveals a positively charged nucleic-acid-binding surface in the RBM25 PWI domain that is entirely different from that in the SRm160 PWI domain Furthermore, we show that the promotion of the pro-apoptotic Bc1-xS isoform expression by RBM25 is facilitated by the PWI domain in vivo. Thus the present study suggests that the PWI domain plays an important role in the regulation of Bcl-x pre-mRNA alternative splicing. |
| 关键词 | alternative splicing Bcl-x pre-mRNA flanking basic region Luc7A PWI domain RNA-binding protein motif 25 (RBM25) |
| 学科领域 | Biochemistry & Molecular Biology |
| DOI | 10.1042/BJ20121382 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | RNA RECOGNITION MOTIF ; BCL-X ; SELENOMETHIONYL PROTEINS ; BINDING DOMAIN ; PHD FINGER ; EXPRESSION ; GENE ; REFINEMENT ; REVEALS ; CANCER |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000314322600009 |
| 出版者 | PORTLAND PRESS LTD |
| 文献子类 | Article |
| 出版地 | LONDON |
| EISSN | 1470-8728 |
| 资助机构 | National Basic Research Program of China(National Basic Research Program of China) ; Chinese National Natural Science Foundation(National Natural Science Foundation of China (NSFC)) |
| 作者邮箱 | Shi, Yunyu/B-3240-2009 |
| 作品OA属性 | Green Published, Green Submitted, hybrid |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/27898 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Mol Physiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, Deshun,Yang, Fan,Li, Fudong,et al. Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region[J]. BIOCHEMICAL JOURNAL,2013,450:85-94. |
| APA | Gong, Deshun.,Yang, Fan.,Li, Fudong.,Qian, Dandan.,Wu, Minhao.,...&Shi, Yunyu.(2013).Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region.BIOCHEMICAL JOURNAL,450,85-94. |
| MLA | Gong, Deshun,et al."Crystal structure and functional characterization of the human RBM25 PWI domain and its flanking basic region".BIOCHEMICAL JOURNAL 450(2013):85-94. |
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| Gong-2013-Crystal st（1476KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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