gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii

doi:10.1007/s00425-013-1938-5

	gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii
	Ling, Yu ; Chen, Tong 1; Jing, Yanping ; Fan, Lusheng 1; Wan, Yinglang ; Lin, Jinxing 1
	2013
发表期刊	PLANTA
ISSN	0032-0935
卷号	238 期号:5 页码:831-843
摘要	gamma-Aminobutyric acid (GABA) is a four-carbon non-protein amino acid found in a wide range of organisms. Recently, GABA accumulation has been shown to play a role in the stress response and cell growth in angiosperms. However, the effect of GABA deficiency on pollen tube development remains unclear. Here, we demonstrated that specific concentrations of exogenous GABA stimulated pollen tube growth in Picea wilsonii, while an overdose suppressed pollen tube elongation. The germination percentage of pollen grains and morphological variations in pollen tubes responded in a dose-dependent manner to treatment with 3-mercaptopropionic acid (3-MP), a glutamate decarboxylase inhibitor, while the inhibitory effects could be recovered in calcium-containing medium supplemented with GABA. Using immunofluorescence labeling, we found that the actin cables were disorganized in 3-MP treated cells, followed by the transition of endo/exocytosis activating sites from the apex to the whole tube shank. In addition, variations in the deposition of cell wall components were detected upon labeling with JIM5, JIM7, and aniline blue. Our results demonstrated that calcium-dependent GABA signaling regulates pollen germination and polarized tube growth in P. wilsonii by affecting actin filament patterns, vesicle trafficking, and the configuration and distribution of cell wall components.
关键词	GABA Pollen tube Calcium Actin filaments Cell wall
学科领域	Plant Sciences
DOI	10.1007/s00425-013-1938-5
收录类别	SCI
语种	英语
WOS关键词	CELL-WALL CONSTRUCTION ; TUBE GROWTH ; ARABIDOPSIS-THALIANA ; UP-REGULATION ; CYTOSKELETON ; MICROTUBULES ; EXPRESSION ; RECEPTORS ; BINDING ; ACCUMULATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326073800003
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-2048
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Key Basic Research Program from MOST ; Program of Introducing Talents of Discipline to Universities (111 project)(Ministry of Education, China - 111 Project) ; Major Science Foundation of Ministry of Education of China ; National Forestry Public Benefit Research Foundation
作者邮箱	Chen, Tong/C-5684-2014
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27902
专题	中科院植物分子生理学重点实验室
作者单位	1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Ling, Yu,Chen, Tong,Jing, Yanping,et al. gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii[J]. PLANTA,2013,238(5):831-843.
APA	Ling, Yu,Chen, Tong,Jing, Yanping,Fan, Lusheng,Wan, Yinglang,&Lin, Jinxing.(2013).gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii.PLANTA,238(5),831-843.
MLA	Ling, Yu,et al."gamma-Aminobutyric acid (GABA) homeostasis regulates pollen germination and polarized growth in Picea wilsonii".PLANTA 238.5(2013):831-843.

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