Knowledge Management System Of Institute Of Botany,CAS
| Proteomic analysis of grape berry skin responding to sunlight exclusion | |
Niu, Ning1,2; Cao, Yuegang1,2; Duan, Wei1; Wu, Benhong1; Li, Shaohua3
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| 2013 | |
| 发表期刊 | JOURNAL OF PLANT PHYSIOLOGY
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| ISSN | 0176-1617 |
| 卷号 | 170期号:8页码:748-757 |
| 摘要 | The most obvious effect of sunlight exclusion from grape clusters is the inhibition of anthocyanin biosynthesis in the berry skin so that no color develops. Two-dimensional gel electrophoresis coupled with mass spectrometry was used to characterize the proteins isolated from berry skins that developed under sunlight exclusion versus those from sunlight-exposed berries. Among more than 1500 spots resolved in stained gels, the accumulation patterns of 96 spots differed significantly between sunlight-excluded berry skin and that of sunlight-exposed control berries. Seventy-two proteins, including 35 down-regulated and 37 up-regulated proteins, were identified and categorized. Proteins involved in photosynthesis and secondary metabolism, especially UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT), the key step for anthocyanin biosynthesis in grape berry skin, were accumulated less in the absence of sunlight. Several isoforms of heat shock proteins were also down-regulated. The proteins that were over-accumulated in sunlight-excluded berry skin were more often related to energy production, glycolysis, the tricarboxylicacid cycle, protein synthesis and biogenesis of cellular components. Their putative role is discussed in terms of their relevance to sunlight exclusion processes. Crown Copyright (C) 2013 Published by Elsevier GmbH. All rights reserved. |
| 关键词 | Anthocyanin Berry skin Grape Proteome analysis Sunlight exclusion |
| 学科领域 | Plant Sciences |
| DOI | 10.1016/j.jplph.2012.12.020 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | VITIS-VINIFERA ; PATHWAY GENES ; EXPRESSION ; FAMILY ; CLONING ; BIOSYNTHESIS ; ACCUMULATION ; RED |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000320297100005 |
| 出版者 | ELSEVIER GMBH |
| 文献子类 | Article |
| 出版地 | MUNICH |
| EISSN | 1618-1328 |
| 资助机构 | CAS Youth Innovation Promotion Association ; National Natural Science Foundation of China (NSFC)(National Natural Science Foundation of China (NSFC)) ; National 948 Project from the Ministry of Agriculture |
| 作者邮箱 | Wu, Benhong/Q-5328-2016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/27917 |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Etiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Ning,Cao, Yuegang,Duan, Wei,et al. Proteomic analysis of grape berry skin responding to sunlight exclusion[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(8):748-757. |
| APA | Niu, Ning,Cao, Yuegang,Duan, Wei,Wu, Benhong,&Li, Shaohua.(2013).Proteomic analysis of grape berry skin responding to sunlight exclusion.JOURNAL OF PLANT PHYSIOLOGY,170(8),748-757. |
| MLA | Niu, Ning,et al."Proteomic analysis of grape berry skin responding to sunlight exclusion".JOURNAL OF PLANT PHYSIOLOGY 170.8(2013):748-757. |
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| 1-s2.0-S017616171300（579KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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