Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli

doi:10.1111/j.1757-1707.2012.01200.x

	Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli
	Kim, Yong-Goo ; Sharmin, Shamima Akhtar ; Alam, Iftekhar ; Kim, Kyung-Hee ; Kwon, Suk-Yoon ; Sohn, Jung-Hoon ; Kim, Sun-Hyung ; Liu, Gongshe3 ; Lee, Byung-Hyun
	2013
发表期刊	GLOBAL CHANGE BIOLOGY BIOENERGY
ISSN	1757-1693
卷号	5 期号:1 页码:73-80
摘要	Reed (Phragmites communis) is a potential bioenergy plant. We report on its first Agrobacterium-mediated transformation using mature seed-derived calli. The Agrobacterium strains LBA4404, EHA105, and GV3101, each harboring the binary vector pIG121Hm, were used to optimize T-DNA delivery into the reed genome. Bacterial strain, cocultivation period and acetosyringone concentration significantly influenced the T-DNA transfer. About 48% transient expression and 3.5% stable transformation were achieved when calli were infected with strain EHA105 for 10 min under 800 mbar negative pressure and cocultivated for 3 days in 200 mu m acetosyringone containing medium. Putative transformants were selected in 25 mg l-1 hygromycin B. PCR, and Southern blot analysis confirmed the presence of the transgenes and their stable integration. Independent transgenic lines contained one to three copies of the transgene. Transgene expression was validated by RT-PCR and GUS staining of stems and leaves.
关键词	Agrobacterium bioenergy biomass Phragmites communis reed transgenic plant
学科领域	Agronomy ; Biotechnology & Applied Microbiology ; Energy & Fuels
DOI	10.1111/j.1757-1707.2012.01200.x
收录类别	SCI
语种	英语
WOS关键词	TALL FESCUE PLANTS ; SUSPENSION-CULTURES ; COMMON REED ; REGENERATION ; DIGESTIBILITY ; PROPAGATION ; VIRULENCE ; RESPONSES ; BARLEY
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000312131100009
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1757-1707
资助机构	KRIBB Research Initiative Program ; Next-Generation BioGreen 21 Program(Rural Development Administration (RDA), Republic of Korea) ; Rural Development Administration(Rural Development Administration (RDA), Republic of Korea) ; Cooperative Research Program for Agricultural Science & Technology Development, RDA, Republic of Korea ; Gyeongsang National University, Republic of Korea
作者邮箱	Kim, Hyung-Kwan/R-8375-2019 ; Sharmin, Shamima/K-4505-2015
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27923
专题	中科院北方资源植物重点实验室
作者单位	1.Gyeongsang Natl Univ, PMBBRC, IALS, Div Appl Life Sci,Program BK21, Jinju 660701, South Korea 2.Korea Res Inst Biosci & Biotechnol, Taejon 305806, South Korea 3.Univ Seoul, Dept Environm Hort, Seoul 130743, South Korea 4.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Kim, Yong-Goo,Sharmin, Shamima Akhtar,Alam, Iftekhar,et al. Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli[J]. GLOBAL CHANGE BIOLOGY BIOENERGY,2013,5(1):73-80.
APA	Kim, Yong-Goo.,Sharmin, Shamima Akhtar.,Alam, Iftekhar.,Kim, Kyung-Hee.,Kwon, Suk-Yoon.,...&Lee, Byung-Hyun.(2013).Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli.GLOBAL CHANGE BIOLOGY BIOENERGY,5(1),73-80.
MLA	Kim, Yong-Goo,et al."Agrobacterium-mediated transformation of reed (Phragmites communis Trinius) using mature seed-derived calli".GLOBAL CHANGE BIOLOGY BIOENERGY 5.1(2013):73-80.

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