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| Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus | |
| Wu, Wang1; Ping, Wenli1; Wu, Hanying; Li, Minchun1; Gu, Dan; Xu, Yinong | |
| 2013 | |
| Source Publication | BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
 |
| ISSN | 0005-2728 |
| Volume | 1827Issue:6Pages:709-722 |
| Abstract | Monogalactosyldiacylglycerol (MGDG) is the most abundant lipid component of the thylakoid membrane. Although MGDG is believed to be important in sustaining the structure and function of the photosynthetic membrane, its exact role in photosynthesis in vivo requires further investigation. In this study, the transgenic tobacco plant M18, which has an MGDG deficiency of approximately 53%, and which contains many fewer thylakoid membranes and exhibits retarded growth and a chlorotic phenotype, was used to investigate the role of MGDG. Chlorophyll fluorescence analysis of the M18 line revealed that PSII activity was inhibited when the plants were exposed to light. The inactive linear electron transport found in M18 plants was mainly attributed to a block in the intersystem electron transport process that was revealed by P700 redox kinetics and PSI light response analysis. Immunoblotting and Blue Native SOS-PAGE analysis suggested that a reduction in the accumulation of cytochrome b(6)f in M18 plants is a direct structural effect of MGDG deficiency, and this is likely to be responsible for the inefficiency observed in intersystem electron transport. Although drastic impairments of PSII subunits were detected in M18 plants grown under normal conditions, further investigations of low-light-grown M18 plants indicated that the impairments are not direct structural effects. Instead, they are likely to result from the cumulative photodamage that occurs due to impaired photostability under long-term exposure to relatively high light levels. The study suggests that MGDG plays important roles in maintaining both the linear electron transport process and the photostability of the PSII apparatus. (c) 2013 Elsevier B.V. All rights reserved. |
| Keyword | Monogalactosyldiacylglycerol Thylakoid membrane Photosystem II Cytochrome b(6)f Electron transport Photoinhibition |
| Subject Area | Biochemistry & Molecular Biology ; Biophysics |
| DOI | 10.1016/j.bbabio.2013.02.013 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ARABIDOPSIS-THALIANA ; CHLOROPHYLL-FLUORESCENCE ; GALACTOLIPID SYNTHESIS ; MEMBRANE-LIPIDS ; SYNTHASE GENES ; INNER ENVELOPE ; B(6)F COMPLEX ; PLANTS ; PHOTOINHIBITION ; PHOTOSYNTHESIS |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000319089400003 |
| Publisher | ELSEVIER SCIENCE BV |
| Subtype | Article |
| Publication Place | AMSTERDAM |
| EISSN | 0006-3002 |
| Funding Organization | National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China(National Basic Research Program of China) |
| OA | Bronze |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/27933 |
| Collection | 中科院光生物学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Beijing Univ, Chinese Acad Sci, Beijing 100049, Peoples R China |
| Recommended Citation GB/T 7714 | Wu, Wang,Ping, Wenli,Wu, Hanying,et al. Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2013,1827(6):709-722. |
| APA | Wu, Wang,Ping, Wenli,Wu, Hanying,Li, Minchun,Gu, Dan,&Xu, Yinong.(2013).Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1827(6),709-722. |
| MLA | Wu, Wang,et al."Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1827.6(2013):709-722. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 23466336.pdf（1708KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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