Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus

doi:10.1016/j.bbabio.2013.02.013

IB-CAS > 中科院光生物学重点实验室

	Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus
	Wu, Wang 1; Ping, Wenli 1; Wu, Hanying ; Li, Minchun 1; Gu, Dan ; Xu, Yinong
	2013
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN	0005-2728
卷号	1827 期号:6 页码:709-722
摘要	Monogalactosyldiacylglycerol (MGDG) is the most abundant lipid component of the thylakoid membrane. Although MGDG is believed to be important in sustaining the structure and function of the photosynthetic membrane, its exact role in photosynthesis in vivo requires further investigation. In this study, the transgenic tobacco plant M18, which has an MGDG deficiency of approximately 53%, and which contains many fewer thylakoid membranes and exhibits retarded growth and a chlorotic phenotype, was used to investigate the role of MGDG. Chlorophyll fluorescence analysis of the M18 line revealed that PSII activity was inhibited when the plants were exposed to light. The inactive linear electron transport found in M18 plants was mainly attributed to a block in the intersystem electron transport process that was revealed by P700 redox kinetics and PSI light response analysis. Immunoblotting and Blue Native SOS-PAGE analysis suggested that a reduction in the accumulation of cytochrome b(6)f in M18 plants is a direct structural effect of MGDG deficiency, and this is likely to be responsible for the inefficiency observed in intersystem electron transport. Although drastic impairments of PSII subunits were detected in M18 plants grown under normal conditions, further investigations of low-light-grown M18 plants indicated that the impairments are not direct structural effects. Instead, they are likely to result from the cumulative photodamage that occurs due to impaired photostability under long-term exposure to relatively high light levels. The study suggests that MGDG plays important roles in maintaining both the linear electron transport process and the photostability of the PSII apparatus. (c) 2013 Elsevier B.V. All rights reserved.
关键词	Monogalactosyldiacylglycerol Thylakoid membrane Photosystem II Cytochrome b(6)f Electron transport Photoinhibition
学科领域	Biochemistry & Molecular Biology ; Biophysics
DOI	10.1016/j.bbabio.2013.02.013
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS-THALIANA ; CHLOROPHYLL-FLUORESCENCE ; GALACTOLIPID SYNTHESIS ; MEMBRANE-LIPIDS ; SYNTHASE GENES ; INNER ENVELOPE ; B(6)F COMPLEX ; PLANTS ; PHOTOINHIBITION ; PHOTOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000319089400003
出版者	ELSEVIER SCIENCE BV
文献子类	Article
出版地	AMSTERDAM
EISSN	0006-3002
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China(National Basic Research Program of China)
作品OA属性	Bronze
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27933
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Beijing Univ, Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wu, Wang,Ping, Wenli,Wu, Hanying,et al. Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2013,1827(6):709-722.
APA	Wu, Wang,Ping, Wenli,Wu, Hanying,Li, Minchun,Gu, Dan,&Xu, Yinong.(2013).Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1827(6),709-722.
MLA	Wu, Wang,et al."Monogalactosyldiacylglycerol deficiency in tobacco inhibits the cytochrome b(6)f-mediated intersystem electron transport process and affects the photostability of the photosystem II apparatus".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1827.6(2013):709-722.

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