Evolutionary divergence of the PISTILLATA-like proteins in Hedyosmum orientale (Chloranthaceae) after gene duplication

doi:10.1111/jse.12045

	Evolutionary divergence of the PISTILLATA-like proteins in Hedyosmum orientale (Chloranthaceae) after gene duplication
	Liu, Shu-Jun ; Du, Xiao-Qiu ; Wu, Feng ; Lin, Xue-Lei ; Xu, Qi-Jing ; Meng, Zheng 5
	2013
发表期刊	JOURNAL OF SYSTEMATICS AND EVOLUTION
ISSN	1674-4918
卷号	51 期号:6 页码:681-692
摘要	Gene duplication and diversification is a significant aspect of gene and genome evolution, even though the evolutionary mechanism following duplication almost certainly varies by genes or gene families. We have identified three PISTILLATA (PI)-like genes, HoPI_1, HoPI_2, and HoPI_3, from basal angiosperm Hedyosmum orientale (Chloranthaceae) and found a functional divergence of these duplicate paralogs in the B-function process. However, the mechanism underlying the divergence of the three HoPI copies remains unclear. In this study, we carefully compared the coding sequence of the three duplicate HoPI paralogs, and investigated their evolutionary process after duplication. Molecular evolutionary analyses suggested that the coding sequence of the relatively recent duplicate paralogs HoPI_1 and HoPI_3 may evolve under purifying selection, but the functional constraint that acted on the coding sequence of HoPI_2 may have been relaxed. Domain swapping and site-directed mutagenesis experiments further showed that HoPI_2 has lost class B-function activity through a gradual process of mutational degradation in MADS and I domains. Although we currently have no direct in vivo evidence that HoPI_2 has lost function in floral development, the relaxed selection, in combination with its inability to rescue pi-1 mutant phenotype, indicate that this distant paralog HoPI_2 might have been converted to be functionless in the B-function process. This study provides a detailed molecular characterization of duplicate PI lineage gene divergence in a single individual level, and our results suggest that mutations in the MI region are more likely to have negative effects on the biochemical activity of PI-like proteins.
关键词	B-function coding sequence divergence gene duplication PISTILLATA-like genes relaxed selection
学科领域	Plant Sciences
DOI	10.1111/jse.12045
收录类别	SCI
语种	英语
WOS关键词	MADS-BOX GENES ; DETECTING POSITIVE SELECTION ; FLORAL HOMEOTIC GENES ; CLASS-B ; FUNCTIONAL DIVERGENCE ; MOLECULAR EVOLUTION ; FLOWER DEVELOPMENT ; COMPLEX PATTERNS ; DOMAIN PROTEINS ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326574200005
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1759-6831
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; Ministry of Science and Technology of China(Ministry of Science and Technology, China)
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27942
专题	中科院植物分子生理学重点实验室
作者单位	1.[Liu, Shu-Jun 2.Du, Xiao-Qiu 3.Wu, Feng 4.Lin, Xue-Lei 5.Xu, Qi-Jing 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.Grad Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Northeast Forestry Univ, Coll Life Sci, Harbin 150040, Peoples R China
推荐引用方式 GB/T 7714	Liu, Shu-Jun,Du, Xiao-Qiu,Wu, Feng,et al. Evolutionary divergence of the PISTILLATA-like proteins in Hedyosmum orientale (Chloranthaceae) after gene duplication[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2013,51(6):681-692.
APA	Liu, Shu-Jun,Du, Xiao-Qiu,Wu, Feng,Lin, Xue-Lei,Xu, Qi-Jing,&Meng, Zheng.(2013).Evolutionary divergence of the PISTILLATA-like proteins in Hedyosmum orientale (Chloranthaceae) after gene duplication.JOURNAL OF SYSTEMATICS AND EVOLUTION,51(6),681-692.
MLA	Liu, Shu-Jun,et al."Evolutionary divergence of the PISTILLATA-like proteins in Hedyosmum orientale (Chloranthaceae) after gene duplication".JOURNAL OF SYSTEMATICS AND EVOLUTION 51.6(2013):681-692.

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