Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes

doi:10.1093/dnares/dss034

	Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes
	Kahloul, Senda 1; El Beji, Imen HajSalah ; Boulaflous, Aurelia ; Ferchichi, Ali 1; Kong, Hongzhi2 ; Mouzeyar, Said ; Bouzidi, Mohamed Fouad
	2013
发表期刊	DNA RESEARCH
ISSN	1340-2838
卷号	20 期号:1 页码:67-78
摘要	The degradation of proteins by the 26S proteasome is initiated by protein polyubiquitination mediated by a three-step cascade. The specific ubiquitination of different target proteins is mediated by different classes of E3 ubiquitin ligases, among which the best known are Skp1-Cullin-F-box complexes. Whereas protists, fungi and some vertebrates have a single SKP1 gene, many animal and plant species possess multiple SKP1 homologues. In this paper, we report on the structure, phylogeny and expression of the complete set of rice SKP1 genes (OSKs, Oryza sativa SKP1-like genes). Our analyses indicated that OSK1 and OSK20 belong to a class of SKP1 genes that contain one intron at a conserved position and are highly expressed. In addition, our yeast two-hybrid results revealed that OSK proteins display a differing ability to interact with F-box proteins. However, OSK1 and OSK20 seemed to interact with most of the nine F-box proteins tested. We suggest that rice OSK1 and OSK20 are likely to have functions similar to the Arabidopsis ASK1 and ASK2 genes.
关键词	SKP1 rice phylogenetic analysis structural analysis yeast two-hybrid screening
学科领域	Genetics & Heredity
DOI	10.1093/dnares/dss034
收录类别	SCI
语种	英语
WOS关键词	SCF E3 COMPLEX ; F-BOX SUBUNIT ; CAENORHABDITIS-ELEGANS ; PROTEIN ; FAMILY ; PLANTS ; CLASSIFICATION ; EVOLUTION ; PATTERNS ; ANIMALS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000315666200006
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1756-1663
资助机构	Tunisian Ministry of Higher Education and Research
作者邮箱	Kong, Hongzhi/B-1784-2012
作品OA属性	Green Submitted, Green Published
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27943
专题	系统与进化植物学国家重点实验室
作者单位	1.Univ Blaise Pascal, Clermont Univ, UMR INRA UBP GDEC 1095, F-63000 Clermont Ferrand, France 2.Lab Aridocultures & Cultures Oasiennes IRA, Medenine 4119, Tunisia 3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Kahloul, Senda,El Beji, Imen HajSalah,Boulaflous, Aurelia,et al. Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes[J]. DNA RESEARCH,2013,20(1):67-78.
APA	Kahloul, Senda.,El Beji, Imen HajSalah.,Boulaflous, Aurelia.,Ferchichi, Ali.,Kong, Hongzhi.,...&Bouzidi, Mohamed Fouad.(2013).Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes.DNA RESEARCH,20(1),67-78.
MLA	Kahloul, Senda,et al."Structural, Expression and Interaction Analysis of Rice SKP1-Like Genes".DNA RESEARCH 20.1(2013):67-78.

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