A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis

doi:10.1016/j.plaphy.2012.12.014

	A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis
	Li, Xiaoxia 1; Gao, Qiong 1; Liang, Ye 1; Ma, Tian 2; Cheng, Liqin ; Qi, Dongmei ; Liu, Hui ; Xu, Xin 2; Chen, Shuangyan ; Liu, Gongshe
	2013
发表期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
卷号	64 页码:52-59
摘要	Salt stress affects plant growth and development, and limits the productivity of crops. Sheepgrass can grow well under various environmental and soil conditions and is a good wild resource in Triticeae. Using 454 high throughout sequencing technique, a large number of salt stress responsive genes have been picked out from sheepgrass. In this study, a novel salt-induced gene and its promoter were cloned and the gene was designated as LcSAIN2 (Leymus chinensis salt-induced 2). Bioinformatics analysis predicted that LcSAIN2 has one transmembrane helix and is localized in nucleus. Experiments of subcellular localization in tobacco leaf cells also indicated that it was mainly localized in nucleus. Several stress responsive elements were found in the promoter region of the LcSAIN2 gene. The expression analysis confirmed that LcSAIN2 was induced by salinity, PEG, ABA, and cold stresses, especially by high salinity. Overexpression of LcSAIN2 in Arabidopsis enhanced salt tolerance of transgenic plants by accumulating osmolytes, such as soluble sugars and free proline, and improving the expression levels of some stress-responsive transcription factors and key genes. Our results suggest that LcSAIN2 might play an important positive modulation role in salt stress tolerance and be a candidate gene utilized for enhancing stress tolerance in wheat and other crops. (C) 2013 Elsevier Masson SAS. All rights reserved.
关键词	LcSAIN2 Novel salt-induced gene Salt tolerance Sheepgrass Arabidopsis
学科领域	Plant Sciences
DOI	10.1016/j.plaphy.2012.12.014
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTIONAL REGULATORY NETWORKS ; FUNCTIONAL-ANALYSIS ; STRESS TOLERANCE ; DROUGHT ; EXPRESSION ; WHEAT ; COLD ; PROLINE ; PROTEIN ; OVEREXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000315547100006
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
文献子类	Article
出版地	ISSY-LES-MOULINEAUX
EISSN	1873-2690
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National High Technology Research and Development Program of China (863)(National High Technology Research and Development Program of China) ; Knowledge Innovation Program of the Chinese Academy of Sciences(Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences) ; Ningxia Agricultural Comprehensive Development Office
作者邮箱	Liu, Huibiao/H-3208-2011
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27977
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Ningxia Univ, Yinchuan, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiaoxia,Gao, Qiong,Liang, Ye,et al. A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2013,64:52-59.
APA	Li, Xiaoxia.,Gao, Qiong.,Liang, Ye.,Ma, Tian.,Cheng, Liqin.,...&Liu, Gongshe.(2013).A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,64,52-59.
MLA	Li, Xiaoxia,et al."A novel salt-induced gene from sheepgrass, LcSAIN2, enhances salt tolerance in transgenic Arabidopsis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 64(2013):52-59.

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