Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics

doi:10.1007/s11120-013-9822-5

	Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics
	Hu, Yuan-Yuan ; Fan, Da-Yong ; Losciale, Pasquale 3,4; Chow, Wah Soon 1; Zhang, Wang-Feng
	2013
发表期刊	PHOTOSYNTHESIS RESEARCH
ISSN	0166-8595
卷号	117 期号:1-3 页码:517-528
摘要	Using radioactively labelled amino acids to investigate repair of photoinactivated photosystem II (PS II) gives only a relative rate of repair, while using chlorophyll fluorescence parameters yields a repair rate coefficient for an undefined, variable location within the leaf tissue. Here, we report on a whole-tissue determination of the rate coefficient of photoinactivation k (i) , and that of repair k (r) in cotton leaf discs. The method assays functional PS II via a P700 kinetics area associated with PS I, as induced by a single-turnover, saturating flash superimposed on continuous background far-red light. The P700 kinetics area, directly proportional to the oxygen yield per single-turnover, saturating flash, was used to obtain both k (i) and k (r) . The value of k (i) , directly proportional to irradiance, was slightly higher when CO2 diffusion into the abaxial surface (richer in stomata) was blocked by contact with water. The value of k (r) , sizable in darkness, changed in the light depending on which surface was blocked by contact with water. When the abaxial surface was blocked, k (r) first peaked at moderate irradiance and then decreased at high irradiance. When the adaxial surface was blocked, k (r) first increased at low irradiance, then plateaued, before increasing markedly at high irradiance. At the highest irradiance, k (r) differed by an order of magnitude between the two orientations, attributable to different extents of oxidative stress affecting repair (Nishiyama et al., EMBO J 20: 5587-5594, 2001). The method is a whole-tissue, convenient determination of the rate coefficient of photoinactivation k (i) and that of repair k (r) .
关键词	Chlorophyll fluorescence P700 Photosystem II Photoinactivation of photosystem II Repair of photosystem II
学科领域	Plant Sciences
DOI	10.1007/s11120-013-9822-5
收录类别	SCI
语种	英语
WOS关键词	PROTEIN-SYNTHESIS ; ELECTRON-FLUXES ; D1 PROTEIN ; PHOTOINHIBITION ; LIGHT ; LEAVES ; PHOTOSYNTHESIS ; DEGRADATION ; PSII ; PHOTODAMAGE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326604900038
出版者	SPRINGER
Special Issue	SI
文献子类	Article
出版地	DORDRECHT
EISSN	1573-5079
资助机构	Australian Research Council(Australian Research Council) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Key Technology R and D Program of China(National Key Technology R&D Program) ; Chinese Academy of Sciences(Chinese Academy of Sciences)
作者邮箱	Losciale, Pasquale/AAE-6191-2021 ; Chow, Wah Soon/D-2010-2009
作品OA属性	Green Submitted
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27979
专题	植被与环境变化国家重点实验室
作者单位	1.Shihezi Univ, Xinjiang Prod & Construct Grp, Key Lab Oasis Ecoagr, Shihezi 832003, Peoples R China 2.Australian Natl Univ, Res Sch Biol, Div Plant Sci, Canberra, ACT 0200, Australia 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 4.Consiglio Ric Sperimentaz Agr, Res Unit Agr Dry Environm, I-70125 Bari, Italy 5.Univ Bologna, Dipartimento Sci Agr, I-40127 Bologna, Italy
推荐引用方式 GB/T 7714	Hu, Yuan-Yuan,Fan, Da-Yong,Losciale, Pasquale,et al. Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics[J]. PHOTOSYNTHESIS RESEARCH,2013,117(1-3):517-528.
APA	Hu, Yuan-Yuan,Fan, Da-Yong,Losciale, Pasquale,Chow, Wah Soon,&Zhang, Wang-Feng.(2013).Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics.PHOTOSYNTHESIS RESEARCH,117(1-3),517-528.
MLA	Hu, Yuan-Yuan,et al."Whole-tissue determination of the rate coefficients of photoinactivation and repair of photosystem II in cotton leaf discs based on flash-induced P700 redox kinetics".PHOTOSYNTHESIS RESEARCH 117.1-3(2013):517-528.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Hu-2013-Whole-tissue（769KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文