Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization

doi:10.1021/pr400291p

	Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization
	Zhao, Xin 1,2; Yang, Ning 1,2; Wang, Tai1
	2013
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	12 期号:11 页码:5058-5071
摘要	In flowering plants, two sperm cells (SCs) are generated from a generative cell (GC) in the developing pollen grain or growing pollen tube and are then delivered to the embryo sac to initiate double fertilization. SC development and function specialization involve the strict control of the protein (gene) expression program and coordination of diverse cellular processes. However, because methods for collecting a large amount of highly purified GCs and SCs for proteomic and transcriptomic studies from a plant are not available, molecular information about the program and the interconnections is lacking. Here, we describe a method for obtaining a large quantity of highly purified GCs and SCs from just-germinated lily pollen grains and growing pollen tubes for proteomic analysis. Our observation showed that SCs had less condensed chromatin and more vacuole-like structures than GCs and that mature SCs were arrested at the G2 phase. Comparison of SC and GC proteomes revealed 101 proteins differentially expressed in the two proteomes. These proteins are involved in diverse cellular and metabolic processes, with preferential involvement in metabolism, the cell cycle, signaling, the ubiquitin/proteasome pathway, and chromatin remodeling. Impressively, almost all proteins in SCF complex-mediated proteolysis and the cell cycle were up-regulated in SCs, whereas those in chromatin remodeling and stress response were down-regulated. Our data also reveal the coordination of SCF complex-mediated proteolysis, cell cycle progression, and DNA repair in SC development and function specialization. This study revealed for the first time a difference in protein profiles between GCs and SCs.
关键词	comparative proteomics proteins pollen grains pollen tubes generative cells sperm cells
学科领域	Biochemical Research Methods
DOI	10.1021/pr400291p
收录类别	SCI
语种	英语
WOS关键词	MALE GAMETOPHYTE DEVELOPMENT ; MALE GAMETIC CELLS ; UBA PROTEIN DDI1 ; F-BOX PROTEIN ; ARABIDOPSIS-THALIANA ; CYCLE PROGRESSION ; LILIUM-LONGIFLORUM ; MATURE POLLEN ; DNA-DAMAGE ; SACCHAROMYCES-CEREVISIAE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000326615000030
出版者	AMER CHEMICAL SOC
文献子类	Article
出版地	WASHINGTON
EISSN	1535-3907
资助机构	Chinese Ministry of Science and Technology(Ministry of Science and Technology, China) ; National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC))
作者邮箱	Wang, Tai/G-2544-2010
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27983
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xin,Yang, Ning,Wang, Tai. Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(11):5058-5071.
APA	Zhao, Xin,Yang, Ning,&Wang, Tai.(2013).Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization.JOURNAL OF PROTEOME RESEARCH,12(11),5058-5071.
MLA	Zhao, Xin,et al."Comparative Proteomic Analysis of Generative and Sperm Cells Reveals Molecular Characteristics Associated with Sperm Development and Function Specialization".JOURNAL OF PROTEOME RESEARCH 12.11(2013):5058-5071.

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