Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica

doi:10.1111/jipb.12046

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	Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica
	Li, Minchun 1; Zhao, Mingming 1; Wu, Hanying ; Wu, Wang 1; Xu, Yinong
	2013
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	55 期号:6 页码:490-503
摘要	Two cDNAs encoding putative type 1 acyl-CoA: diacylglycerol acyltransferases (DGAT1, EC 2.3.1.20), were cloned from Tetraena mongolica, an extreme xerophyte with high oil content in the stems. The 1 488-bp and 1 485-bp of the open reading frame (ORF) of the two cDNAs, designated as TmDGAT1a and TmDGAT1b, were both predicted to encode proteins of 495 and 494 amino acids, respectively. Southern blot analysis revealed that TmDGAT1a and TmDGAT1b both had low copy numbers in the T. mongolica genome. In addition to ubiquitous expression with different intensity in different tissues, including stems, leaves and roots, TmDGAT1a and TmDGAT1b, were found to be strongly induced by high salinity, drought and osmotic stress, resulting in a remarkable increase of triacylglycerol (TAG) accumulation in T. mongolica plantlets. TmDGAT1a and TmDGAT1b activities were confirmed in the yeast H1246 quadruple mutant (DGA1, LRO1, ARE1, ARE2) by restoring DGAT activity of the mutant host to produce TAG. Overexpression of TmDGAT1a and TmDGAT1b in soybean hairy roots as well as in T. mongolica calli both resulted in an increase in oil content (ranging from 37% to 108%), accompanied by altered fatty acid profiles.
关键词	Diacylglycerol acyltransferase1 Tetraena mongolica triacylglycerol
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/jipb.12046
收录类别	SCI
语种	英语
WOS关键词	BRASSICA-NAPUS ; OIL CONTENT ; TRIACYLGLYCEROL SYNTHESIS ; ENDOPLASMIC-RETICULUM ; LIPID-SYNTHESIS ; FATTY-ACID ; EXPRESSION ; ENZYME ; BIOSYNTHESIS ; TOBACCO
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000320276600002
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	National Natural Science Foundation of China(National Natural Science Foundation of China (NSFC)) ; National Basic Research Program of China(National Basic Research Program of China)
引用统计	被引频次：19[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27989
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Minchun,Zhao, Mingming,Wu, Hanying,et al. Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2013,55(6):490-503.
APA	Li, Minchun,Zhao, Mingming,Wu, Hanying,Wu, Wang,&Xu, Yinong.(2013).Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,55(6),490-503.
MLA	Li, Minchun,et al."Cloning, Characterization and Functional Analysis of Two Type 1 diacylglycerol acyltransferases (DGAT1s) from Tetraena mongolica".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 55.6(2013):490-503.

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